PLGA nanofibers improves the antitumoral effect of daunorubicin

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 136; pp. 248 - 255
• Main Authors: Guimarães, Pedro P.G., Oliveira, Michele F., Gomes, Alinne D.M., Gontijo, Sávio M.L., Cortés, Maria E., Campos, Paula P., Viana, Celso T.R., Andrade, Silvia P., Sinisterra, Rubén D.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-12-2015
• Subjects: Animals; Antibiotics, Antineoplastic - pharmacology; Biomedical materials; Cell Line; Cell Line, Tumor; Daunorubicin; Daunorubicin - pharmacology; Differential scanning calorimetry; Differential thermal analysis; Drug delivery system; Electrospinning; Humans; In vitro testing; In vivo testing; In vivo tests; Lactic Acid - chemistry; Male; Mice; Microscopy, Electron, Scanning; Microscopy, Fluorescence; Nanofibers; PLGA nanofibers; Polyglycolic Acid - chemistry; Surgical implants; X-Ray Diffraction; Electrospinning; PLGA nanofibers; Daunorubicin; Drug delivery system
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2015.09.005

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•PLGA nanofibers loaded with daunorubicin (PLGA-DNR) were prepared by electrospinning process.•Sixty-five percent of the DNR was released in an initial burst over 8h and by 1224h, eighty-five percent of the DNR had been released, modelled by zero order kinetics.•In vitro experiments demonstrated that the PLGA-DNR nanofibers had a higher cytotoxic effect to A431 tumor cells than free DNR.•In vivo data showed that the nanofibers had an antiangiogenic effect, no inflammatory potential and decreased of the vascularization.
The objective of this study was to evaluate the in vivo anti-inflammatory angiogenesis activity and in vitro cytotoxicity on normal and cancer cell models of a drug delivery system consisting of poly(lactic-co-glycolic acid) nanofibers loaded with daunorubicin (PLGA-DNR) that were fabricated using an electrospinning process. The PLGA-DNR nanofibers were also characterized by thermogravimetric analysis (TGA), differential thermal analysis (DTA) and differential scanning calorimetry (DSC), X-ray diffraction (XRD), scanning electron microscopy (SEM) and confocal fluorescence microscopy. In vitro release of DNR from the nanofibers and its corresponding mechanism were also evaluated. Sixty-five percent of the DNR was released in an initial burst over 8h, and by 1224h, eighty-five percent of the DNR had been released. The Higuchi model yielded the best fit to the DNR release profile over the first 8h, and the corresponding data from 24 to 1224h could be modeled using zero-order kinetics. The PLGA-DNR nanofibers exhibited a higher cytotoxicity to A431 cells than free DNR but a cytotoxicity similar to free DNR against fibroblast cells. A higher antiangiogenic effect of PLGA nanofibers was observed in the in vivo data when compared to free DNR, and no inflammatory potential was observed for the nanofibers.