Structural characterization, antiproliferative and immunoregulatory activities of a polysaccharide from Boletus Leccinum rugosiceps

• Published in: International journal of biological macromolecules Vol. 157; pp. 106 - 118
• Main Authors: Li, Yimeng, You, Lijun, Dong, Fang, Yao, Wanzi, Chen, Jian
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15-08-2020
• Subjects: Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; Antitumor; Basidiomycota - chemistry; Carbohydrate Sequence; Cell Proliferation - drug effects; Fungal Polysaccharides - chemistry; Fungal Polysaccharides - pharmacology; Hep G2 Cells; Humans; Immunologic Factors - chemistry; Immunologic Factors - pharmacology; Immunomodulatory; Leccinum rugosiceps; MCF-7 Cells; Polysaccharide; Structure; Immunomodulatory; Structure; Polysaccharide; Antitumor; Leccinum rugosiceps
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2020.03.250

A homogeneous water-soluble flaky polysaccharide named LRP-1 (18.82 kDa) was extracted and purified from the rare edible and medicinal fungi Leccinum rugosiceps. The structure characterization of LRP-1 was analyzed by GPC, GC, GC–MS, FT-IR, SEM, 1D/2D NMR, and its antitumor and immunoregulatory activities were determined. The results show that LRP-1 is a new polysaccharide with a backbone which is mainly composed of →1-α-D-Fucp-3→, →1-α-D-Rhap-3→, →1-β-D-Glcp-6→, →1-β-D-Glcp-3→, →1-α-D-Glcp-4→, →1-β-D-Galp-6 → and →1-α-D-Manp-6→. Results of MTT assay show that LRP-1 inhibit the growth of human hepatoma cells HepG-2 and human breast carcinoma MCF-7 cells in vitro. LRP-1 could also induce the secretion of NO and immune factors (IL-6 and TNF-α) of RAW264.7. This study suggests that LRP-1 has potential to be used as nutraceutical food with antitumor and immunoregulatory activities.