Retesting and follow-up of first-catch urines from men yield variable results with three Chlamydia trachomatis nucleic acid amplification tests

• Published in: APMIS : acta pathologica, microbiologica et immunologica Scandinavica Vol. 108; no. 11; pp. 725 - 728
• Main Authors: NordbØ, SVEIN ARNE, Lund, KRISTIN, Skjeldestad, FINN EGIL
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01-11-2000; Blackwell
• Subjects: Adolescent; Adult; Bacterial diseases; Bacterial diseases of the urinary system; Bacteriuria - diagnosis; Biological and medical sciences; Chlamydia Infections - diagnosis; Chlamydia trachomatis; Chlamydia trachomatis - genetics; Chlamydia trachomatis - isolation & purification; Human bacterial diseases; Humans; Infectious diseases; LCR; Ligase Chain Reaction; Male; Medical sciences; Nucleic Acid Amplification Techniques; PCR; Polymerase Chain Reaction; Reproducibility of Results; TMA; urine; Urine - microbiology; Performance evaluation; Human; Urinary system disease; Chlamydiaceae; Male; Chlamydia trachomatis; Medical screening; Infection; Polymerase chain reaction; Transcription mediated amplification; Ligase chain reaction; Bacteriosis; Chlamydiales; Bacteria; Chlamydiosis; Diagnosis; Comparative study
• ISSN: 0903-4641; 1600-0463
• DOI: 10.1034/j.1600-0463.2000.d01-20.x

First‐catch urines from 276 asymptomatic male military recruits were screened by polymerase chain reaction for the detection of Chlamydia trachomatis. Eight initially positive specimens were retested by polymerase chain reaction, ligase chain reaction and transcription‐mediated amplification. Urine specimens from six (2.2%) subjects were considered to contain C. trachomatis. However, retesting of serially collected urines from five of these six subjects using different nucleic acid amplification methods showed some discrepancy. This may have a major impact on the efficacy of screening programs for C trachomatis in low prevalence populations.