Immunohistochemical expression of β-catenin, E-cadherin, cyclin D1 and c-myc in benign trichogenic tumors

• Published in: Journal of cutaneous pathology Vol. 34; no. 6; pp. 467 - 473
• Main Authors: Demirkan, Neşe Çallı, Bir, Ferda, Erdem, Özlem, Düzcan, Ender
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01-06-2007; Blackwell
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; beta Catenin - metabolism; Biological and medical sciences; Biomarkers, Tumor - metabolism; Cadherins - metabolism; Child; Child, Preschool; Cyclin D; Cyclins - metabolism; Dermatology; Female; Hair Diseases - metabolism; Hair Diseases - pathology; Humans; Immunohistochemistry; Male; Medical sciences; Middle Aged; Neoplasm Proteins - metabolism; Pilomatrixoma - metabolism; Pilomatrixoma - pathology; Proto-Oncogene Proteins c-myc - metabolism; Skin Neoplasms - metabolism; Skin Neoplasms - pathology; Immunohistochemistry; Anatomic pathology; Dermatology; C-Onc gene; β Catenin; myc Gene; Cyclin E; Benign neoplasm; E Cadherin
• ISSN: 0303-6987; 1600-0560
• DOI: 10.1111/j.1600-0560.2006.00636.x

Background:  β‐catenin gene mutations have been reported in vast majority of pilomatrixomas (PMXs). β‐catenin, a component of the adhesion molecules of the cytoskeleton, is degraded at the cytoplasm. Excess cytoplasmic β‐catenin enters into the nucleus and activates the transcription of several genes encoding c‐myc, cyclin D1 and others. Sublocation of β‐catenin has been demonstrated by immunohistochemistry. The aim of this study was to determine the role of β‐catenin‐related proteins in various benign trichogenic tumors.
Methods:  We investigated the expression of β‐catenin, E‐cadherin, c‐myc and cyclin D1 immunohistochemically, and the expression of these molecules were compared between two groups consisting of 12 PMXs and 12 other benign trichogenic tumors (OBTTs).
Results:  In PMX group, nuclear and/or cytoplasmic expression of β‐catenin was associated with a loss of membranous expression of E‐cadherin (p = 0.002). In OBTT group, a membranous expression of E‐cadherin and β‐catenin was observed, and there was a stronger nuclear immunoreactivity of cyclin D1 compared with PMX group (p = 0.006).
Conclusions:  In PMX, nuclear and/or cytoplasmic β‐catenin expression of tumoral cells is not related with β‐catenin‐related gene expressions (c‐myc or cyclin D1). The molecular behaviour of OBTTs is clearly different from that of PMXs in terms of to E‐cadherin and β‐catenin expression.