Cone photoreceptor preservation with laser photobiomodulation in murine and human retinal dystrophy

• Published in: Clinical and translational medicine Vol. 12; no. 2; pp. e673 - n/a
• Main Authors: Casson, Robert J., Wood, John P. M., Ao, Jack, Gilhotra, Jagjit S., Durkin, Shane R., Muecke, James, Chan, WengOnn, Chidlow, Glyn
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01-02-2022; John Wiley and Sons Inc; Wiley
• Subjects: Animals; Bioenergetics; Cytochrome; Disease; Disease Models, Animal; Gene therapy; Genetic engineering; Humans; Investigations; Lasers; Letter to Editor; Light emitting diodes; Low-Level Light Therapy - instrumentation; Low-Level Light Therapy - methods; Low-Level Light Therapy - statistics & numerical data; Mice; Oxidative stress; Photoreceptors; Retina; Retinal Cone Photoreceptor Cells; Retinal Dystrophies - physiopathology; Retinal Dystrophies - therapy; Visual acuity
• ISSN: 2001-1326; 2001-1326
• DOI: 10.1002/ctm2.673

Bioenergetic dysfunction and oxidative stress are implicated in the pathogenesis of secondary cone degeneration in RP;1,2 and are mitigated by the photonic action of PBM on cytochrome c oxidase in the electron transport chain.3 To date, PBM research on the retina has almost invariably used light-emitting diode (LED) systems; however, this methodology suffers the disadvantage that the energy delivery at the level of the retina is uncontrolled. [...]using qPCR and western blotting, we showed the elevated expression of photoreceptor genes as well as antioxidant genes in PBM-treated samples (Figure S2C). [...]we assessed residual vision by recording the optokinetic reflex at P35 and observed partial preservation of this visual reflex in the PBM-treated groups (Figure 2A).