Effect of beta-adrenergic receptor antagonism on chloralose-induced hemodynamic changes in newborn lambs

• Published in: Journal of cardiovascular pharmacology Vol. 20; no. 6; p. 990
• Main Authors: Covert, R F, Schreiber, M D, Torgerson, L J
• Format: Journal Article
• Language: English
• Published: United States 01-12-1992
• Subjects: Adrenergic beta-Antagonists - pharmacology; Animals; Animals, Newborn - physiology; Cardiac Pacing, Artificial; Chloralose - pharmacology; Heart Rate - drug effects; Hemodynamics - drug effects; Isoproterenol - pharmacology; Oxygen Consumption - drug effects; Pulmonary Circulation - drug effects; Respiratory Function Tests; Sheep; Ventricular Function
• ISSN: 0160-2446
• DOI: 10.1097/00005344-199212000-00021

alpha-Chloralose is an anesthetic commonly used in cardiovascular research. Using a chronically instrumented neonatal lamb model, we previously determined that chloralose has important effects on basal hemodynamics and arterial oxygen tension as compared with those of paired conscious control lambs. We wished to determine whether beta-adrenergic receptor stimulation accounted for chloralose-induced hemodynamic effects and to investigate the influence of chloralose and beta-adrenergic receptor antagonism on oxygen metabolism. In paired studies, five lambs were given chloralose intravenously (30 mg/kg i.v.) after propranolol (1 mg/kg i.v.) or saline control. The group pretreated with propranolol had reduced heart rate (HR 206 +/- 12 vs. 244 +/- 10 beats/min, p = 0.04) and cardiac output (CO 253 +/- 29 vs. 302 +/- 40 ml/min/kg, p = 0.005) 30 min after chloralose as compared with control; pretreatment with propranolol also attenuated the systemic hypertensive response to chloralose (77 +/- 8 vs. 89 +/- 5 mm Hg, p = 0.055). No difference in the response of stroke volume (SV), atrial or pulmonary arterial pressures, or pulmonary and systemic vascular resistances (PVR, SVR) were observed between treatment groups. No differences between propranolol and saline treatment groups were observed in arterial and mixed venous oxygen contents, arteriovenous (A-V) oxygen difference, oxygen extraction, or oxygen consumption; a reduction in oxygen delivery observed after propranolol as compared with saline was not altered by chloralose. We conclude that tachycardia and increase in CO induced by chloralose in lambs probably are mediated by beta-adrenergic receptor stimulation, which may be direct or indirect.