Transplantation of SNAP-treated adipose tissue-derived stem cells improves cardiac function and induces neovascularization after myocardium infarct in rats

• Published in: Experimental and molecular pathology Vol. 90; no. 2; pp. 149 - 156
• Main Authors: Berardi, Gel R.M., Rebelatto, Carmen K., Tavares, Heloísa F., Ingberman, Max, Shigunov, Patrícia, Barchiki, Fabiane, Aguiar, Alessandra M., Miyague, Nelson I., Francisco, Julio C., Correa, Alejandro, Senegaglia, Alexandra C., Suss, Paula Hansen, Moutinho, José A., Sotomaior, Vanessa S., Nakao, Lia S., Brofman, Paulo S.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01-04-2011; Elsevier
• Subjects: Adipose Tissue - cytology; Adipose-derived stem cells; Animals; Biological and medical sciences; Cardiomyogenesis; Ejection fraction; Glutathione - metabolism; Green Fluorescent Proteins - metabolism; Heart - drug effects; Heart - physiopathology; Heart Function Tests - drug effects; Intracellular Space - drug effects; Intracellular Space - metabolism; Investigative techniques, diagnostic techniques (general aspects); Male; Medical sciences; Myocardial Infarction - physiopathology; Myocardial Infarction - therapy; Neovascularization; Neovascularization, Physiologic - drug effects; Nitric oxide; Nitrosation - drug effects; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Rats; Rats, Wistar; S-Nitroso-N-Acetylpenicillamine - pharmacology; SNAP; Stem Cell Transplantation; Stem Cells - cytology; Stem Cells - drug effects; Stem Cells - metabolism; Stroke Volume - drug effects; Troponin - metabolism; von Willebrand Factor - metabolism; Cardiomyogenesis; Adipose-derived stem cells; Neovascularization; SNAP; Nitric oxide; Ejection fraction; Cardiac performance; Cell function; Adipose tissue; Infarct; Rat; SNAP protein; Stem cell; Rodentia; Transplantation; Vertebrata; Anatomic pathology; Mammalia; Treatment; Animal; Myocardium; Circulatory system
• ISSN: 0014-4800; 1096-0945
• DOI: 10.1016/j.yexmp.2010.11.005

Stem cell therapy has been considered a promise for damaged myocardial tissue. We have previously shown that S-nitroso-N-acetyl-D,L-penicillamine (SNAP) increases the expression of several muscular markers and VEGF in mesenchymal stem cells, indicating that transplantation of SNAP-treated cells could provide better functional outcomes. Here, we transplanted SNAP-treated adipose tissue-derived stem cells (ADSCs) in rat infarcted myocardium. After 30days, we observed a significant improvement of the ejection fraction in rats that received SNAP-treated ADSCs, compared with those that received untreated cells (p=0.008). Immunohistochemical reactions showed an increased expression of troponin T–C and von Willebrand factor, and organized vascular units in the infarcted area of tissue transplanted with treated ADSCs. SNAP exposure induced intracellular S-nitrosation, a decreased GSH/GSSG ratio, but did not increase cGMP levels. Collectively, these results indicate that SNAP alters the redox environment of ADSCs, possibly associated with a pre-differentiation state, which may improve cardiac function after transplantation.