Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems

• Published in: Brazilian archives of biology and technology Vol. 46; no. 4; pp. 741 - 750
• Main Authors: Yan, Xu(Hebei Institute of Technology Department of Chemical Engineering), Souza, Maria Aparecida(FCF/USP Biochemical and Pharmaceutical Technology Department), Pontes, Marcela Z. R.(FCF/USP Biochemical and Pharmaceutical Technology Department), Vitolo, Michele(FCF/USP Biochemical and Pharmaceutical Technology Department), Pessoa Júnior, Adalberto(FCF/USP Biochemical and Pharmaceutical Technology Department)
• Format: Journal Article
• Language: English
• Published: Tecpar 01-12-2003; Instituto de Tecnologia do Paraná (Tecpar)
• Subjects: Affinity partitioning; Aqueous two-phase systems; Enzymes; Ligands; Reactive dyes
• ISSN: 1516-8913; 1516-8913; 1678-4324
• DOI: 10.1590/s1516-89132003000400030

From analytical to commercial scale, aqueous two-phase systems have their application in the purification, characterization and study of biomaterials. In order to improve the selectivity of the systems, the biospecific affinity ligands were introduced. In the affinity partitioning aqueous two-phase system, have many enzymes been purified. This review discusses the partitioning of some enzymes in the affinity aqueous two-phase systems in regard to the different ligands, including reactive dyes, metal ions and other ligands. Some integration of aqueous two-phase system with other techniques for more effective purification of enzymes are also presented.
Tanto em escala de laboratório como industrial, os sistemas de duas fases aquosas podem ser utilizados para a purificação, caracterização e estudos de biomateriais. Para aumentar a seletividade desse sistema, ligantes de afinidade bioespecíficos podem ser utilizados. No sistema de duas fases aquosas por afinidade, muitas enzimas podem ser purificadas. Neste artigo de revisão, a partição de algumas enzimas por esse tipo de afinidade, utilizando diferentes ligantes como corantes e íons metálicos, são discutidas. Além disso, a integração desse sistema de duas fases aquosas com outras técnicas de purificação estão sendo apresentados, com o objetivo mostrar a melhoria da eficiência do processo.