Genetic transformation of Trametes versicolor to phleomycin resistance with the dominant selectable marker shble

• Published in: Applied microbiology and biotechnology Vol. 56; no. 1/2; pp. 201 - 204
• Main Authors: Bartholomew, K, Dos Santos, G, Dumonceaux, T, Charles, T, Archibald, F
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01-07-2001; Springer Nature B.V
• Subjects: Anti-Bacterial Agents - pharmacology; Basidiomycetes; Basidiomycota - drug effects; Basidiomycota - genetics; Biological and medical sciences; Biotechnology; Blotting, Southern; calcium chloride; Coriolus versicolor; Deoxyribonucleic acid; DNA; Drug Resistance, Fungal; Fundamental and applied biological sciences. Psychology; Genetic engineering; Genetic technics; genetic transformation; Genetics; Methods. Procedures. Technologies; Microbiology; Miscellaneous; phleomycin; Phleomycins - pharmacology; polyethylene; polyethylene glycol; protoplast fusion; Schizophyllum commune; Synthetic digonucleotides and genes. Sequencing; Trametes versicolor; Transformation, Genetic; Fungi; Ethylene oxide polymer; Recombinant microorganism; Plasmid; Resistance; Genetic transformation; Transcription promoter; Protoplast; Selection; Coriolus versicolor; Basidiomycetes; Thallophyta
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s002530100635

We have developed a stable, DNA-mediated transformation system for the white-rot basidiomycete Trametes versicolor based on the dominant selectable marker shble (phleomycin resistance). We employed a vector containing the selectable marker under control of expression sequences from the basidiomycete Schizophyllum commune and a polyethylene glycol/CaCl2 protoplast-fusion technique to introduce the transforming DNA. This transformation system generated stable phleomycin-resistant transformants at a frequency of four to seven transformants/microgram of transforming DNA.