Identification of proteins differentially expressed in the conventional renal cell carcinoma by proteomic analysis

• Published in: Journal of Korean medical science Vol. 20; no. 3; pp. 450 - 455
• Main Authors: Hwa, Jeong Seok, Park, Hyo Jin, Jung, Jae Hun, Kam, Sung Chul, Park, Hyung Chul, Kim, Choong Won, Kang, Kee Ryeon, Hyun, Jea Seog, Chung, Ky Hyun
• Format: Journal Article
• Language: English
• Published: Korea (South) The Korean Academy of Medical Sciences 01-06-2005; 대한의학회
• Subjects: Aged; Aldehyde Reductase - analysis; alpha 1-Antitrypsin - analysis; Amidohydrolases - analysis; Carcinoma, Renal Cell - metabolism; Carcinoma, Renal Cell - pathology; Electrophoresis, Gel, Two-Dimensional; Enoyl-CoA Hydratase - analysis; Female; Fructokinases - analysis; Humans; Kidney Neoplasms - metabolism; Kidney Neoplasms - pathology; Male; Middle Aged; Original; Proteome - analysis; Proteomics - methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Tropomyosin - analysis; Ureohydrolases - analysis; Vimentin - analysis; 의학일반
• ISSN: 1011-8934; 1598-6357
• DOI: 10.3346/jkms.2005.20.3.450

Renal cell carcinoma (RCC) is one of the most malignant tumors in urology, and due to its insidious onset patients frequently have advanced disease at the time of clinical presentation. Thus, early detection is crucial in management of RCC. To identify tumor specific proteins of RCC, we employed proteomic analysis. We prepared proteins from conventional RCC and the corresponding normal kidney tissues from seven patients with conventional RCC. The expression of proteins was determined by silver stain after two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). The overall protein expression patterns in the RCC and the normal kidney tissues were quite similar except some areas. Of 66 differentially expressed protein spots (p<0.05 by Student t-test), 8 different proteins from 11 spots were identified by MALDI-TOF-MS. The expression of the following proteins was repressed (p<0.05); aminoacylase-1, enoyl-CoA hydratase, aldehyde reductase, tropomyosin alpha-4 chain, agmatinase and ketohexokinase. Two proteins, vimentin and alpha-1 antitrypsin precursor, were dominantly expressed in RCC (p<0.05).