Genetic and cytological diversity in cherry tree accessions ( Eugenia involucrata DC) in Rio Grande do Sul

• Published in: Crop breeding and applied biotechnology Vol. 16; no. 3; pp. 219 - 225
• Main Authors: Guerra, Divanilde, Souza, Paulo Vitor Dutra de, Schwarz, Sérgio Francisco, Schifino-Wittmann, Maria Teresa, Werlang, Claudio André, Veit, Pedro Augusto
• Format: Journal Article
• Language: English
• Published: Vicosa Crop Breeding and Applied Biotechnology 01-09-2016; Brazilian Society of Plant Breeding
• Subjects: AGRONOMY; Anthers; BIOTECHNOLOGY & APPLIED MICROBIOLOGY; Breeding; cytogenetic analysis; Deoxyribonucleic acid; Diploids; DNA; Flowers; Fruit trees; Gene polymorphism; Genetic diversity; Germination; Hybridization; hybridizations; In vitro methods and tests; Meiosis; molecular analysis; Plant diversity; Plantations; Pollen; Polymerase chain reaction; Polymorphism; Random amplified polymorphic DNA; Similarity; Stability analysis; Trees; Viability; Rio Grande do Sul Brazil; hybridizations; cytogenetic analysis; Breeding; molecular analysis
• ISSN: 1984-7033; 1518-7853; 1984-7033
• DOI: 10.1590/1984-70332016v16n3a33

This study aimed to evaluate the genetic and cytological diversity and stability of 35 cherry tree accessions collected in Rio Grande do Sul. We used 15 RAPD (Random Amplified Polymorphic DNA) molecular markers and performed cytological analysis and number count of anthers. Analyses of genetic diversity allowed the separation of accessions into four groups, resulting in an average of 8.93 bands per primer amplified, 7.89 polymorphic bands, 88.08% of polymorphism and 86% of genetic similarity. Cytological analyses of gametic cells allowed for the characterization of accessions as diploids with n=11. In these, the average of meiotic cells considered normal was 82.12%; average pollen viability was 92.44% and in vitro germination was 40.26%; the average number of anthers was 161.85 anthers/flowers. Therefore, the accessions evaluated showed high genetic similarity and cytological stability and can be used in commercial plantations or hybridizations.