Effect of Microbial Species Richness on Community Stability and Community Function in a Model Plant-Based Wastewater Processing System

• Published in: Microbial ecology Vol. 52; no. 4; pp. 725 - 737
• Main Authors: Cook, K. L, Garland, J. L, Layton, A. C, Dionisi, H. M, Levine, L. H, Sayler, G. S
• Format: Journal Article
• Language: English
• Published: New York, NY New York : Springer-Verlag 01-11-2006; Springer Science + Business Media, Inc; Springer; Springer Nature B.V
• Subjects: Activated sludge; Bacteria; Bacteria - genetics; Bacteria - growth & development; Bacteriology; Biodiversity; Biological and medical sciences; Biosensors; Carbon sources; Community; Competition; Cultured cells; DNA, Ribosomal - genetics; Environmental Microbiology; Fundamental and applied biological sciences. Psychology; Industrial wastewater; Industrial wastewater treatment; Invasive species; Microbiology; Microorganisms; Miscellaneous; Plant roots; Plants; Plants - microbiology; Pseudomonas fluorescens; Rhizosphere; Ribosomal DNA; RNA, Ribosomal, 16S - genetics; RNA, Ribosomal, 23S - genetics; Sewage - microbiology; Species; Species diversity; Species richness; Surfactants; Waste Disposal, Fluid - methods; Wastewater; Wastewater treatment; Wastewater treatment plants; Water purification; Pseudomonadales; Activated sludge; Oxygen; Purification; Waste treatment; Ribosomal DNA; Pseudomonas fluorescens; Waste water; Rhizosphere; Biosensor; Bacteria; Pseudomonadaceae; Isolate; Models; Plant growth promoting rhizobacteria; Microorganism; Recycling; Waste water purification; Microbial community
• ISSN: 0095-3628; 1432-184X
• DOI: 10.1007/s00248-006-9105-1

Microorganisms will be an integral part of biologically based waste processing systems used for water purification or nutrient recycling on long-term space missions planned by the National Aeronautics and Space Administration. In this study, the function and stability of microbial inocula of different diversities were evaluated after inoculation into plant-based waste processing systems. The microbial inocula were from a constructed community of plant rhizosphere-associated bacteria and a complexity gradient of communities derived from industrial wastewater treatment plant-activated sludge. Community stability and community function were defined as the ability of the community to resist invasion by a competitor (Pseudomonas fluorescens 5RL) and the ability to degrade surfactant, respectively. Carbon source utilization was evaluated by measuring surfactant degradation and through Biolog and BD oxygen biosensor community level physiological profiling. Community profiles were obtained from a 16S-23S rDNA intergenic spacer region array. A wastewater treatment plant-derived community with the greatest species richness was the least susceptible to invasion and was able to degrade surfactant to a greater extent than the other complexity gradient communities. All communities resisted invasion by a competitor to a greater extent than the plant rhizosphere isolate constructed community. However, the constructed community degraded surfactant to a greater extent than any of the other communities and utilized the same number of carbon sources as many of the other communities. These results demonstrate that community function (carbon source utilization) and community stability (resistance to invasion) are a function of the structural composition of the community irrespective of species richness or functional richness.