Genome-Wide Identification of TLP Gene Family in Populus trichocarpa and Functional Characterization of PtTLP6 , Preferentially Expressed in Phloem

• Published in: International journal of molecular sciences Vol. 25; no. 11; p. 5990
• Main Authors: Guo, Mengjie, Ma, Xujun, Xu, Shiying, Cheng, Jiyao, Xu, Wenjing, Elsheery, Nabil Ibrahim, Cheng, Yuxiang
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01-06-2024; MDPI
• Subjects: Amino acids; Antifungal agents; Arabidopsis - genetics; Arabidopsis - metabolism; Chemical bonds; Chromosomes; early flowering; Flowers - genetics; Flowers - metabolism; Fungi; Gene Expression Regulation, Plant; Genes; Genome, Plant; Genomes; Genomics; Multigene Family; peroxisome; Phloem - genetics; Phloem - metabolism; Phylogenetics; Phylogeny; Plant Proteins - genetics; Plant Proteins - metabolism; Plant resistance; Plants, Genetically Modified - genetics; Populus - genetics; Populus - metabolism; Populus trichocarpa; Proteins; reactive oxygen species; Reactive Oxygen Species - metabolism; TLP; China; TLP; peroxisome; early flowering; Populus trichocarpa; reactive oxygen species
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25115990

Thaumatin-like proteins (TLPs) in plants are involved in diverse biotic and abiotic stresses, including antifungal activity, low temperature, drought, and high salinity. However, the roles of the
genes are rarely reported in early flowering. Here, the
gene family was identified in
. The 49
genes were classified into 10 clusters, and gene structures, conserved motifs, and expression patterns were analyzed in these
genes. Among 49
genes, the
transcription level is preferentially high in stems, and GUS staining signals were mainly detected in the phloem tissues of the
pro::GUS transgenic poplars. We generated transgenic
plants overexpressing the
gene, and its overexpression lines showed early flowering phenotypes. However, the expression levels of main flowering regulating genes were not significantly altered in these
-overexpressing plants. Our data further showed that overexpression of the
gene led to a reactive oxygen species (ROS) burst in
, which might advance the development process of transgenic plants. In addition, subcellular localization of
-fused green fluorescent protein (GFP) was in peroxisome, as suggested by tobacco leaf transient transformation. Overall, this work provides a comprehensive analysis of the
gene family in
and an insight into the role of TLPs in woody plants.