Nasal eosinophilia and eosinophil peroxidase in children and adolescents with rhinitis

• Published in: Clinical and experimental pediatrics Vol. 62; no. 9; pp. 353 - 359
• Main Authors: Choi, Yeonu, Jeon, Haeun, Yang, Eun Ae, Yoon, Jong-Seo, Kim, Hyun Hee
• Format: Journal Article
• Language: English
• Published: Korea (South) Clinical and Experimental Pediatics / Korean Pediatric Society 01-09-2019; Korean Pediatric Society; 대한소아청소년과학회
• Subjects: Child; Eosinophil; Eosinophil peroxidase; Inflammation; Original; Proteins; Rhinitis; Sinuses; Sinusitis; Statistical analysis; 소아과학; United States > US; Eosinophil peroxidase; Rhinitis; Child; Eosinophil
• ISSN: 1738-1061; 2092-7258; 2713-4148
• DOI: 10.3345/kjp.2019.00318

Researcher showed that EPO is a relatively accurate marker of eosinophilia and eosinophil activity and that EPO has a higher correlation to them. However, it's use as markers of eosinophilic inflammation in nasal secretions is limited because the diagnostic cut-off values of EPO for use as one-time tests for allergic diseases such as allergic rhinitis have not been established.
To identify the correlation between nasal eosinophil and eosinophil peroxidase (EPO) in children and adolescents with rhinitis.
We recruited patients below 18 years of age with rhinitis for more than two weeks or recurring more than twice a year whose nasal eosinophil and EPO were measured at the Allergy Clinic of a single, The eosinophil percentage was calculated by dividing the eosinophil count by the number of total cells with a light microscope at 1000× magnification. EPO and protein were measured from nasal secretions. We analyzed the correlation between nasal eosinophils and protein-corrected EPO (EPO/protein) value, retrospectively.
Of the 67 patients enrolled, Forty-one subjects were males (61.2%) and the mean age was 8.2 years ± 4.0. The median value of nasal eosinophil count was 1 and percentage was 1%. The median protein-corrected EPO value was 12.5 ng/μg (0 to 31 ng/μg). There was a statistically significant correlation between the eosinophil count and percentage (P <0.001). However, the eosinophil percentage and EPO did not correlate. The eosinophil count and EPO had a statistically significant correlation (P =0.01). The EPO cut-off value examined for nasal eosinophil counts were 2, 5, 10, and 20 was 17.57 ng/μg regardless of the reference count. The largest area under curve (AUC) value was obtained when the receiver operating characteristic (ROC) curve was drawn using the eosinophil count of 2.
The nasal eosinophil count was significantly associated with protein-corrected EPO.