Genotyping of clinical Rhodotorula mucilaginosa isolates by pulsed field gel electrophoresis
Summary Identification and typing of fungal isolates is a prerequisite for control and prevention of nosocomial infections. As the discriminatory power of phenotypic methods is not sufficient for epidemiological purposes, genotyping methods such as DNA fingerprinting, random amplification of polymor...
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| Published in: | Mycoses Vol. 46; no. 11-12; pp. 487 - 491 |
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| Main Authors: | , , , , |
| Format: | Journal Article |
| Language: | English |
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Berling, Germany
Blackwell Verlag, GmbH
01-12-2003
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| Abstract | Summary
Identification and typing of fungal isolates is a prerequisite for control and prevention of nosocomial infections. As the discriminatory power of phenotypic methods is not sufficient for epidemiological purposes, genotyping methods such as DNA fingerprinting, random amplification of polymorphic DNA (RAPD) analysis, or pulsed field gel electrophoresis (PFGE) are preferred. To our knowledge, this study is the first application of PFGE for typing Rhodotorula mucilaginosa strains. The PFGE patterns of six clinical isolates produced two different karyotypes, which were confirmed by RAPD analysis. Five strains isolated from bloodstream infections from three different institutions showed the same karyotype and RAPD patterns, while the urological specimen differed slightly.
Zusammenfassung
Identifizierung und Typisierung von Pilzisolaten sind Voraussetzung für Bekämpfung und Verhinderung nosokomialer Infektionen. Da phänotypische Methoden für epidemiologische Zwecke nicht ausreichend diskriminierend sind, werden Genotypierungsmethoden wie zum Beispiel Plasmid‐Fingerprinting, RAPD und PFGE bevorzugt. Diese Arbeit ist die erstmalige Typisierung von Rhodotorula mucilaginosa mittels PFGE. Das PFGE‐Muster von sechs klinisch isolierten Keimen ergab zwei verschiedene Karyotypen, die auch durch verschiedene RAPD‐Muster bestätigt wurden. Alle fünf Keime, welche aus der Blutbahn isoliert wurden, zeigten das gleiche genotypische Muster, nur wenig abweichend von denen urologischer Herkunft. |
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| AbstractList | Summary
Identification and typing of fungal isolates is a prerequisite for control and prevention of nosocomial infections. As the discriminatory power of phenotypic methods is not sufficient for epidemiological purposes, genotyping methods such as DNA fingerprinting, random amplification of polymorphic DNA (RAPD) analysis, or pulsed field gel electrophoresis (PFGE) are preferred. To our knowledge, this study is the first application of PFGE for typing Rhodotorula mucilaginosa strains. The PFGE patterns of six clinical isolates produced two different karyotypes, which were confirmed by RAPD analysis. Five strains isolated from bloodstream infections from three different institutions showed the same karyotype and RAPD patterns, while the urological specimen differed slightly.
Zusammenfassung
Identifizierung und Typisierung von Pilzisolaten sind Voraussetzung für Bekämpfung und Verhinderung nosokomialer Infektionen. Da phänotypische Methoden für epidemiologische Zwecke nicht ausreichend diskriminierend sind, werden Genotypierungsmethoden wie zum Beispiel Plasmid‐Fingerprinting, RAPD und PFGE bevorzugt. Diese Arbeit ist die erstmalige Typisierung von Rhodotorula mucilaginosa mittels PFGE. Das PFGE‐Muster von sechs klinisch isolierten Keimen ergab zwei verschiedene Karyotypen, die auch durch verschiedene RAPD‐Muster bestätigt wurden. Alle fünf Keime, welche aus der Blutbahn isoliert wurden, zeigten das gleiche genotypische Muster, nur wenig abweichend von denen urologischer Herkunft. Identification and typing of fungal isolates is a prerequisite for control and prevention of nosocomial infections. As the discriminatory power of phenotypic methods is not sufficient for epidemiological purposes, genotyping methods such as DNA fingerprinting, random amplification of polymorphic DNA (RAPD) analysis, or pulsed field gel electrophoresis (PFGE) are preferred. To our knowledge, this study is the first application of PFGE for typing Rhodotorula mucilaginosa strains. The PFGE patterns of six clinical isolates produced two different karyotypes, which were confirmed by RAPD analysis. Five strains isolated from bloodstream infections from three different institutions showed the same karyotype and RAPD patterns, while the urological specimen differed slightly. Identifizierung und Typisierung von Pilzisolaten sind Voraussetzung für Bekämpfung und Verhinderung nosokomialer Infektionen. Da phänotypische Methoden für epidemiologische Zwecke nicht ausreichend diskriminierend sind, werden Genotypierungsmethoden wie zum Beispiel Plasmid‐Fingerprinting, RAPD und PFGE bevorzugt. Diese Arbeit ist die erstmalige Typisierung von Rhodotorula mucilaginosa mittels PFGE. Das PFGE‐Muster von sechs klinisch isolierten Keimen ergab zwei verschiedene Karyotypen, die auch durch verschiedene RAPD‐Muster bestätigt wurden. Alle fünf Keime, welche aus der Blutbahn isoliert wurden, zeigten das gleiche genotypische Muster, nur wenig abweichend von denen urologischer Herkunft. Identification and typing of fungal isolates is a prerequisite for control and prevention of nosocomial infections. As the discriminatory power of phenotypic methods is not sufficient for epidemiological purposes, genotyping methods such as DNA fingerprinting, random amplification of polymorphic DNA (RAPD) analysis, or pulsed field gel electrophoresis (PFGE) are preferred. To our knowledge, this study is the first application of PFGE for typing Rhodotorula mucilaginosa strains. The PFGE patterns of six clinical isolates produced two different karyotypes, which were confirmed by RAPD analysis. Five strains isolated from bloodstream infections from three different institutions showed the same karyotype and RAPD patterns, while the urological specimen differed slightly. Identification and typing of fungal isolates is a prerequisite for control and prevention of nosocomial infections. As the discriminatory power of phenotypic methods is not sufficient for epidemiological purposes, genotyping methods such as DNA fingerprinting, random amplification of polymorphic DNA (RAPD) analysis, or pulsed field gel electrophoresis (PFGE) are preferred. To our knowledge, this study is the first application of PFGE for typing Rhodotorula mucilaginosa strains. The PFGE patterns of six clinical isolates produced two different karyotypes, which were confirmed by RAPD analysis. Five strains isolated from bloodstream infections from three different institutions showed the same karyotype and RAPD patterns, while the urological specimen differed slightly.Original Abstract: Identifizierung und Typisierung von Pilzisolaten sind Voraussetzung fuer Bekaempfung und Verhinderung nosokomialer Infektionen. Da phaenotypische Methoden fuer epidemiologische Zwecke nicht ausreichend diskriminierend sind, werden Genotypierungsmethoden wie zum Beispiel Plasmid-Fingerprinting, RAPD und PFGE bevorzugt. Diese Arbeit ist die erstmalige Typisierung von Rhodotorula mucilaginosa mittels PFGE. Das PFGE-Muster von sechs klinisch isolierten Keimen ergab zwei verschiedene Karyotypen, die auch durch verschiedene RAPD-Muster bestaetigt wurden. Alle fuenf Keime, welche aus der Blutbahn isoliert wurden, zeigten das gleiche genotypische Muster, nur wenig abweichend von denen urologischer Herkunft. |
| Author | Sener, K. Wickes, B. L. Saracli, M. A. Yildiran, S. T. Gonlum, A. |
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| Cites_doi | 10.1001/archinte.1969.00300110080016 10.1111/j.1439-0507.1996.tb00509.x 10.1080/mmy.38.s1.139.145 10.1080/00362177685190481 10.1080/00362176785190201 10.1016/B978-0-12-482850-6.50009-5 10.1053/jhin.2001.1023 10.1136/hrt.37.1.101 10.1099/00222615-39-3-161 10.1016/S0163-4453(88)94097-2 10.1128/iai.60.11.4872-4880.1992 10.1128/CMR.9.4.499 10.1099/00207713-47-2-432 10.1080/00362176285190051 10.1080/00362177785380071 10.1046/j.1439-0507.2000.00562.x 10.1111/j.1468-3083.1999.tb01010.x 10.1099/00222615-5-4-391 10.1046/j.1365-2958.2001.02375.x 10.1093/clinids/11.3.369 10.1128/CMR.13.2.332-370.2000 10.1099/00221287-140-3-543 10.1086/647644 10.1016/0002-9343(83)91216-0 |
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| References_xml | – volume: 15 start-page: 251 year: 1977 end-page: 6 article-title: Colonization of hospitalized patients with yeast‐like organisms publication-title: Sabouraudia – volume: 11 start-page: 369 year: 1989 end-page: 78 article-title: New spectrum of fungal infections in patients with cancer publication-title: Rev Infect Dis – volume: 49 start-page: 37 year: 2001 end-page: 42 article-title: Rate of transmission and endogenous origin of and on adult intensive care units studied by pulsed field gel electrophoresis publication-title: J Hosp Infect – volume: 18 start-page: 426 year: 1997 end-page: 38 article-title: How to select and interpret molecular strain typing methods for epidemiological studies of bacterial infections: a review for healthcare epidemiologists publication-title: Infect Control Hosp Epidemiol – volume: 86 start-page: 111 year: 2001 article-title: infection in a kidney transplant patient with pancytopenia publication-title: Haematologica – volume: 16 start-page: 187 year: 1988 end-page: 91 article-title: ventriculitis publication-title: J Infect – volume: 14 start-page: 331 year: 1976 end-page: 5 article-title: Meningitis caused by publication-title: Sabouraudia – volume: 75 start-page: 349 year: 1983 end-page: 52 article-title: peritonitis in patients undergoing continuous ambulatory peritoneal dialysis publication-title: Am J Med – volume: 39 start-page: 161 year: 1993 end-page: 2 article-title: PCR fingerprinting microbes by random amplification of polymorphic DNA publication-title: J Med Microbiol – volume: 47 start-page: 432 year: 1997 end-page: 42 article-title: Molecular typing of : taxonomic and epidemiological aspects publication-title: Int J Syst Bacteriol – volume: 39 start-page: 73 issue: Suppl.1 year: 1996 end-page: 80 article-title: Anwendung molekularbiologischer Methoden für Diagnostik und Epidemiologie humaner Pilzinfektionen publication-title: Mycoses – volume: 140 start-page: 543 year: 1994 end-page: 50 article-title: Comparison of the electrophoretic karyotypes and chromosomal location of ten genes in the two varieties of publication-title: Microbiology – volume: 38 start-page: 139 issue: Suppl. 1 year: 2000 end-page: 45 article-title: Conventional versus molecular diagnostic tests publication-title: Medical Mycology – volume: 13 start-page: 332 year: 2000 end-page: 70 article-title: The ins and outs of DNA fingerprinting the infectious fungi publication-title: Clin Microbiol Rev – volume: 5 start-page: 391 year: 1972 end-page: 4 article-title: The isolation of red yeast‐like fungi in a diagnostic laboratory publication-title: J Med Microbiol – volume: 5 start-page: 110 year: 1966 end-page: 9 article-title: Speciation and densities of yeasts in human urine specimens publication-title: Sabouraudia – volume: 123 start-page: 78 year: 1969 end-page: 81 article-title: fungemia presenting as “endotoxic” shock publication-title: Arch Intern Med – volume: 43 start-page: 209 year: 2000 end-page: 10 article-title: Case report. fungemia due to use of indwelling venous catheters publication-title: Mycoses – volume: 1 start-page: 8 year: 1961 end-page: 15 article-title: Yeasts from human sources publication-title: Sabouraudia – start-page: 768 year: 1992 end-page: 82 – volume: 12 start-page: 169 year: 1999 end-page: 70 article-title: fungemia in an immunosupressed patient publication-title: J Eur Acad Venereol – volume: 60 start-page: 4872 year: 1992 end-page: 80 article-title: Electrophoretic karyotypes of clinical isolates of publication-title: Infect Immun – start-page: 337 year: 1980 end-page: 410 – volume: 9 start-page: 499 year: 1996 end-page: 511 article-title: Epidemiology of nosocomial fungal infections publication-title: Clin Microbiol Rev – volume: 37 start-page: 101 year: 1975 end-page: 14 article-title: Endocarditis caused by successfully treated with 5‐fluorocytosine publication-title: Br Heart J – volume: 40 start-page: 200 year: 2001 end-page: 213 article-title: The gene is alpha‐specific and required for mating publication-title: Molec Microbiol – volume: 123 start-page: 78 year: 1969 ident: e_1_2_9_12_2 article-title: Rhodotorula fungemia presenting as “endotoxic” shock publication-title: Arch Intern Med doi: 10.1001/archinte.1969.00300110080016 contributor: fullname: Leeber DA – ident: e_1_2_9_14_2 doi: 10.1111/j.1439-0507.1996.tb00509.x – ident: e_1_2_9_15_2 doi: 10.1080/mmy.38.s1.139.145 – ident: e_1_2_9_10_2 doi: 10.1080/00362177685190481 – ident: e_1_2_9_6_2 doi: 10.1080/00362176785190201 – ident: e_1_2_9_17_2 doi: 10.1016/B978-0-12-482850-6.50009-5 – ident: e_1_2_9_25_2 doi: 10.1053/jhin.2001.1023 – ident: e_1_2_9_9_2 doi: 10.1136/hrt.37.1.101 – ident: e_1_2_9_24_2 doi: 10.1099/00222615-39-3-161 – ident: e_1_2_9_7_2 doi: 10.1016/S0163-4453(88)94097-2 – volume: 60 start-page: 4872 year: 1992 ident: e_1_2_9_26_2 article-title: Electrophoretic karyotypes of clinical isolates of Coccidioides immitis publication-title: Infect Immun doi: 10.1128/iai.60.11.4872-4880.1992 contributor: fullname: Pan S – ident: e_1_2_9_20_2 doi: 10.1128/CMR.9.4.499 – ident: e_1_2_9_27_2 doi: 10.1099/00207713-47-2-432 – ident: e_1_2_9_4_2 doi: 10.1080/00362176285190051 – ident: e_1_2_9_3_2 doi: 10.1080/00362177785380071 – ident: e_1_2_9_21_2 doi: 10.1046/j.1439-0507.2000.00562.x – ident: e_1_2_9_23_2 doi: 10.1111/j.1468-3083.1999.tb01010.x – ident: e_1_2_9_5_2 doi: 10.1099/00222615-5-4-391 – ident: e_1_2_9_19_2 doi: 10.1046/j.1365-2958.2001.02375.x – ident: e_1_2_9_11_2 doi: 10.1093/clinids/11.3.369 – start-page: 768 volume-title: Medical Mycology year: 1992 ident: e_1_2_9_2_2 contributor: fullname: Kwon‐Chung KJ – ident: e_1_2_9_13_2 doi: 10.1128/CMR.13.2.332-370.2000 – ident: e_1_2_9_18_2 doi: 10.1099/00221287-140-3-543 – ident: e_1_2_9_16_2 doi: 10.1086/647644 – ident: e_1_2_9_8_2 doi: 10.1016/0002-9343(83)91216-0 – volume: 86 start-page: 111 year: 2001 ident: e_1_2_9_22_2 article-title: Rhodotorula rubra infection in a kidney transplant patient with pancytopenia publication-title: Haematologica contributor: fullname: Navarro JT |
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| Snippet | Summary
Identification and typing of fungal isolates is a prerequisite for control and prevention of nosocomial infections. As the discriminatory power of... Identification and typing of fungal isolates is a prerequisite for control and prevention of nosocomial infections. As the discriminatory power of phenotypic... |
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| SubjectTerms | DNA Fingerprinting - methods Electrophoresis, Gel, Pulsed-Field Fungemia - microbiology Genotype genotyping Genotypisierung Humans Karyotyping - methods Male Molecular Epidemiology - methods Mycological Typing Techniques - methods Mycoses - microbiology Prostate - microbiology pulsed field gel electrophoresis Pulsfeld-Gelektrophorese Random Amplified Polymorphic DNA Technique - methods Rhodotorula - classification Rhodotorula - genetics Rhodotorula - isolation & purification Rhodotorula mucilaginosa Turkey |
| Title | Genotyping of clinical Rhodotorula mucilaginosa isolates by pulsed field gel electrophoresis |
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