In vitro antioxidant activity of anthocyanins of black soybean seed coat in human low density lipoprotein (LDL)

• Published in: Food chemistry Vol. 112; no. 3; pp. 659 - 663
• Main Authors: Astadi, Ignasius Radix, Astuti, Mary, Santoso, Umar, Nugraheni, Prihati Sih
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01-02-2009; [Amsterdam]: Elsevier Science; Elsevier
• Subjects: Anthocyanin; anthocyanins; antioxidant activity; Biological and medical sciences; Black soybean; cultivars; DPPH; food analysis; food composition; Food industries; free radical scavengers; Fruit and vegetable industries; Fundamental and applied biological sciences. Psychology; Glycine max; in vitro studies; LDL; low density lipoprotein; Phenolic; phenolic compounds; soybeans; TBARS; testa; Anthocyanin; Black soybean; Phenolic; TBARS; DPPH; LDL; Human; Seeds; Soybean; Antioxidant; Flavonoid; In vitro; Lipoprotein LDL; Grain legume; Polyphenol; Pigments; Phenols
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2008.06.034

The objectives of this study were to determine the phenolic and anthocyanin contents in black soybean
Mallika and
Cikuray variety seed coat extract and to examine antioxidant activity of extract against DPPH radical and LDL oxidation. Black soybean seed coat of
Mallika (M) and
Cikuray (C) was extracted using methanol-1%HCl. The phenolic and anthocyanin contents were determined with Folin–Ciocalteu and pH differential methods, respectively. Individual anthocyanidins were identified with HPLCdiode array detector, and antioxidant activity was examined, using DPPH and TBARS assay with LDL as the oxidation substrate. BHT and rutin were used as antioxidant references. The phenolic content in M and C were 8.15
±
0.23 and 6.46
±
0.11
g
GAE/100
g, respectively. The anthocyanin contents were 11.36
±
0.12 and 1.45
±
0.13
g/100
g, respectively. Cyanidin, delphinidin, and pelargonidin were found as individual anthocyanidins. The optimum DPPH radical scavenging capacity (%) of M and C were 92.78% and 91.50%, respectively, BHT and rutin were 77.0% and 91.94%, respectively. The optimum inhibition of TBARS formation from M and C were 37.10 and 30.37
nmol MDA equivalents/g LDL protein, respectively, and rutin were 30.10
nmol MDA equivalents/g LDL protein, respectively. These results suggest that black soybean seed coat has high levels of phenolic and anthocyanin, and also demonstrated considerable antioxidant activity of black soybean seed coat.