Sex steroids level in blood plasma and ovarian follicles of the chimeric chicken

• Published in: Journal of veterinary medicine. Series A Vol. 53; no. 10; p. 501
• Main Authors: Sechman, A, Lakota, P, Wojtysiak, D, Hrabia, A, Mika, M, Lisowski, M, Czekalski, P, Rzasa, J, Kapkowska, E, Bednarczyk, M
• Format: Journal Article
• Language: English
• Published: Germany Hindawi Limited 01-12-2006
• Subjects: Animals; Blood; Chick Embryo; Chickens - blood; Chickens - metabolism; Chimera; Estradiol - analysis; Estradiol - blood; Female; Gonadal Steroid Hormones - analysis; Gonadal Steroid Hormones - blood; Granulosa Cells - metabolism; Hormones; Ovarian Follicle - enzymology; Ovarian Follicle - metabolism; Oviposition - physiology; Plasma; Poultry; Progesterone - analysis; Progesterone - blood; Steroids; Testosterone - analysis; Testosterone - blood; Theca Cells - metabolism
• ISSN: 0931-184X; 1865-1674; 1865-1682
• DOI: 10.1111/j.1439-0442.2006.00886.x

The study was performed to determine the hormonal status of mature germline chimeras obtained by blastodermal cell transfer from chicken embryos of a donor breed [Green-legged Partridgelike breed (GP) x Araucana (AR)] to those of a recipient breed [White Leghorn (WL)] being at the same stage of embryonic development. The egg-laying chimeras and WL hens (control) of the same age were used in the experiment. At first, blood samples were taken from each bird at 0.5, 5, 12.5 and 18.5 h following oviposition. Subsequently, the chimeras and the WL hens were decapitated 1-2 h after ovulation. A stroma and the following follicles were isolated from the ovary: white normal (1-4, 4-6 and 6-8 mm), white atretic and yellow preovulatory follicles (F4-F1). Sex hormones, progesterone (P4), testosterone (T) and oestradiol (E2) in blood plasma and ovarian follicles were determined radioimmunologically. The activity of the 3beta-hydroxysteroid dehydrogenase (3beta-HSD) in the granulosa and theca layers of the follicles was analysed histochemically. In chimeric chickens, a higher level of T in blood plasma during the ovulatory cycle was noticed. However, in the stroma, white prehierarchical and medium-size preovulatory ovarian follicles the level of T was significantly lower. With respect to E2, its elevated levels were found both in blood and in the ovarian follicles. There were no significant differences in P4 concentrations in blood plasma while in ovarian follicles a higher level was observed only in white 6-8 mm follicles. 3beta-HSD activity in granulosa and theca layers of the ovarian follicles in chimeras was not different from that in the WL hens. In conclusion, the results obtained indicate that germline chimeras exhibit significant alterations in sex hormone levels in the ovary and blood plasma, which in turn may affect their reproductive abilities.