Anti-metastatic and anti-angiogenic activities of sulfated polysaccharide of Sepiella maindroni ink

• Published in: Carbohydrate polymers Vol. 91; no. 1; pp. 403 - 409
• Main Authors: Zong, Aizhen, Zhao, Ting, Zhang, Yan, Song, Xinlei, Shi, Yikang, Cao, Hongzhi, Liu, Chunhui, Cheng, Yanna, Qu, Xianjun, Cao, Jichao, Wang, Fengshan
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 02-01-2013
• Subjects: Angiogenesis; Angiogenesis Inhibitors - chemistry; Angiogenesis Inhibitors - pharmacology; Angiogenesis Inhibitors - therapeutic use; Animals; Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; Antineoplastic Agents - therapeutic use; Cell Line, Tumor; Decapodiformes - chemistry; Fibroblast Growth Factor 2 - genetics; Fibroblast Growth Factor 2 - metabolism; Gene Expression Regulation, Neoplastic - drug effects; Humans; ICAM-1; Intercellular Adhesion Molecule-1 - genetics; Intercellular Adhesion Molecule-1 - metabolism; Melanoma metastasis; Melanoma, Experimental - blood supply; Melanoma, Experimental - genetics; Melanoma, Experimental - metabolism; Melanoma, Experimental - pathology; Mice; Neoplasm Metastasis; Neovascularization, Pathologic - drug therapy; Pigmentation; Polysaccharides - chemistry; Polysaccharides - pharmacology; Polysaccharides - therapeutic use; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sepiella maindroni ink; Sulfated polysaccharide; Sulfates - chemistry; Angiogenesis; Sepiella maindroni ink; ICAM-1; Melanoma metastasis; Sulfated polysaccharide
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2012.08.050

► Previous study demonstrated that SIP-SII had antimetastatic activity in vitro. ► SIP-SII significantly inhibited melanoma metastasis in vivo. ► SIP-SII inhibited angiogenesis in chick chorioallantoic membrane assay. ► SIP-SII inhibited the expression of ICAM-1 and bFGF both in vitro and in vivo.
A previous study demonstrated that SIP-SII, a sulfated Sepiella maindroni ink polysaccharide, suppressed the invasion and migration of cancer cells via the inhibition of the proteolytic activity of matrix metalloproteinase-2 (MMP-2). Therefore, this study investigated the anti-metastatic effect of SIP-SII in vivo. SIP-SII (15 and 30mg/kgd) markedly decreased B16F10 pulmonary metastasis in mice models by 85.9% and 88.0%, respectively. Immunohistochemistry showed that SIP-SII decreased the expression of the intercellular adhesion molecule 1 (ICAM-1) and basic fibroblast growth factor (bFGF) in lung metastasis nodules. In addition, SIP-SII inhibited neovascularization in chick chorioallantoic membrane assay at 0.08–2mg/mL. In the in vitro experiments, SIP-SII (0.8–500μg/mL) significantly decreased the protein and mRNA expression of ICAM-1 and bFGF in SKOV3 and EA.hy926 cells, respectively. These results suggested that SIP-SII might suppress melanoma metastasis via the inhibition of the tumor adhesion mediated by ICAM-1 and the angiogenesis mediated by bFGF, as well as resulting in depression of the invasion and migration of carcinoma cells.