Impact of Protease Inhibitors on Dentin Matrix Degradation by Collagenase

• Published in: Journal of dental research Vol. 91; no. 12; pp. 1119 - 1123
• Main Authors: Kato, M.T., Leite, A.L., Hannas, A.R., Calabria, M.P., Magalhães, A.C., Pereira, J.C., Buzalaf, M.A.R.
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA SAGE Publications 01-12-2012; SAGE PUBLICATIONS, INC
• Subjects: Analysis of Variance; Animals; Bacterial Proteins - metabolism; Cariostatic Agents - therapeutic use; Catechin - analogs & derivatives; Catechin - therapeutic use; Cattle; Chlorhexidine; Chlorhexidine - therapeutic use; Citric acid; Collagen; Collagen - metabolism; Collagenase; Collagenases - metabolism; Dentin; Dentin - metabolism; Dentin - ultrastructure; Dentistry; Enzymes; Epigallocatechin-3-gallate; Extracellular Matrix - metabolism; Ferrous Compounds - therapeutic use; Fluorides; Gels; Hydroxyproline; Protease Inhibitors - therapeutic use; Proteinase inhibitors; Saliva; Sodium Fluoride - therapeutic use; Spectrum analysis; Studies; Tooth Demineralization - enzymology; Tooth Demineralization - prevention & control; Tooth Erosion - enzymology; Tooth Erosion - prevention & control; United States > US; dentin; enzyme inhibitors; matrix metalloproteinases; tooth erosion; extracellular matrix; hydroxyproline
• ISSN: 0022-0345; 1544-0591
• DOI: 10.1177/0022034512455801

This proof-of-concept study assessed whether the reduction of the degradation of the demineralized organic matrix (DOM) by pre-treatment with protease inhibitors (PI) is effective against dentin matrix loss. Bovine dentin slices were demineralized with 0.87 M citric acid, pH 2.3, for 36 hrs. In sequence, specimens were treated or not (UT, untreated) for 1 min with gels containing epigallocatechin 3-gallate (EGCG, 400 µM), chlorhexidine (CHX, 0.012%), FeSO4 (1 mM), NaF (1.23%), or no active compound (P, placebo). Specimens were then stored in artificial saliva (5 days, 37°C) with the addition of collagenase (Clostridium histolyticum, 100 U/mL). We analyzed collagen degradation by assaying hydroxyproline (HYP) in the incubation solutions (n = 5) and evaluated the dentin matrix loss by profilometry (n = 12). Data were analyzed by ANOVA and Tukey’s test (p < 0.05). Treatment with gels containing EGCG, CHX, or FeSO4 led to significantly lower HYP concentrations in solution and dentin matrix loss when compared with the other treatments. These results strongly suggest that the preventive effects of the PI tested against dentin erosion are due to their ability to reduce the degradation of the DOM.