Construction and structure-activity mechanism of polysaccharide nano-selenium carrier

• Published in: Carbohydrate polymers Vol. 236; p. 116052
• Main Authors: Hu, Shuqian, Hu, Weicheng, Li, Yanru, Li, Shujing, Tian, Huafeng, Lu, Ang, Wang, Jianguo
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 15-05-2020
• Subjects: Anti-inflammatory activity; Polysaccharides; Selenium nanoparticles; Structure-activity relationship; Anti-inflammatory activity; Polysaccharides; Structure-activity relationship; Selenium nanoparticles
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2020.116052

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•The water solubility of polysaccharides was improved by sulfation modification.•The water-soluble sulfated polysaccharides were used as stabilizer.•Size-controlled and stable nano-selenium particles were prepared via one-step way.•Size-dependent bioactivities of the prepared nano-selenium were evaluated.
In this study, a series water soluble sulfate polysaccharides (SPS) with different degrees of substitutions (DS = 0.02∼0.28) were prepared using a linear water-insoluble β-d-(1→3)-glucan. SPS-1, SPS-3 and SPS-7 with substitution degrees of 0.02, 0.06 and 0.25 were used as templates to prepare stable and size controlled selenium nanoparticles (SeNPs) with diameter from 54.35 to 123.04 nm using one-step method. The selenium contents of SPS-SeNPs with large, medium and small sizes were 0.172 %, 0.274 % and 0.305 %, respectively. SPS-SeNPs was confirmed to inhibit the production of nitric oxide (NO) in RAW 264.7 inflammatory macrophages induced by lipopolysaccharide (LPS), and downregulated the mRNA expression of TNF-α, IL-1β and iNOS. The results indicated that SPS-SeNPs had significant anti-inflammatory activity. Moreover, SPS-SeNPs with smaller size showed higher anti-inflammatory effects. All of these results suggest that SeNPs-SPS carriers have anti-inflammatory in concentration-dependent and size-dependent model.