Effect of Saccharomyces boulardii and Bacillus cereus var. toyoi on the humoral and cellular response of mice to vaccines

• Published in: Food and Agricultural Immunology Vol. 16; no. 1-4; pp. 213 - 219
• Main Authors: Coppola, M. de M, Conceicao, F.R, Gil-Turnes, C
• Format: Journal Article
• Language: English
• Published: London Taylor & Francis Group 01-09-2005; Taylor and Francis
• Subjects: antibody formation; Bacillus cereus; Bacillus cereus biovar toyoi; Bacillus cereus var toyoi; bacterial antigens; Biological and medical sciences; Canine parvovirus; cell-mediated immunity; Escherichia coli; Feline panleukopenia virus; Food industries; Food microbiology; Fundamental and applied biological sciences. Psychology; Fundamental immunology; humoral immunity; immune-modulation; immunization; interferons; interleukin-4; mice; oral administration; Parvovirus; Probiotics; Saccharomyces; Saccharomyces boulardii; seroconversion; vaccination; vaccines; Probiotic; Saccharomyces boulardii; immunemodulation; Rodentia; Bacillaceae; Bacillus cereus var toyoi; Fungi; Probiotics; Vertebrata; Mammalia; Bacillus cereus; Bacillales; Mouse; Bacteria; Thallophyta
• ISSN: 0954-0105; 1465-3443; 1365-2567
• DOI: 10.1080/09540100500244138

The effect of Saccharomyces boulardii and Bacillus cereus var. toyoi on the humoral and cellular response of mice to the simultaneous inoculation of a tetravalent E. coli bacterin and a viable vaccine against Canine Parvovirus is reported. Thirty-six isogenic BALB-c female mice, seven weeks old, were vaccinated with 1/20th of the dose used for pigs and dogs, respectively, and randomly divided into three groups. Group 1 received non-supplemented feed, group 2 was supplemented with S. boulardii, and group 3 with Bacillus cereus var. toyoi. The humoral response was quantified through ELISAs using specific antigens, and the cellular response quantifying IL-4 and IFNg through ELISA. Means of seroconversions to the bacterin were always higher in supplemented mice than in controls, varying from 1.6-1.8 for group 2 and from 1.2-1.4 for group 3. Seroconversions against Parvovirus for group 2 were 5.2-12 times higher, and those of group 3 were 6.8-9.1 times higher than those of controls. IL-4 was produced by spleen cells of S. boulardii supplemented mice stimulated with E. coli fimbriae.