Effect of vitrification on meiotic maturation and expression of genes in immature goat cumulus oocyte complexes

• Published in: Cryobiology Vol. 64; no. 3; pp. 176 - 184
• Main Authors: Rao, Brahmasani Sambasiva, Mahesh, Yelisetti Uma, Charan, Katari Venu, Suman, Komjeti, Sekhar, Nithya, Shivaji, Sisinthy
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Inc 01-06-2012; Elsevier
• Subjects: Animals; Apoptosis; Apoptosis Regulatory Proteins - genetics; Apoptosis Regulatory Proteins - metabolism; Bax protein; Biological and medical sciences; Bone morphogenetic protein receptor type II; Cell Survival - drug effects; Cells, Cultured; Cryopreservation; Cryopreservation - methods; Cryoprotective Agents - pharmacology; Cumulus Cells - cytology; Cumulus Cells - drug effects; Cumulus Cells - metabolism; Dimethyl sulfoxide; Dimethyl Sulfoxide - pharmacology; double prime CS gene; Ethylene glycol; Ethylene Glycol - pharmacology; Female; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation - drug effects; Goat; Goats; Growth Differentiation Factors - genetics; Growth Differentiation Factors - metabolism; Immature oocyte; Mammalian reproduction. General aspects; Meiosis; Meiosis - drug effects; Meiosis - genetics; Oocyte secreted factors; Oocytes; Oocytes - cytology; Oocytes - drug effects; Oocytes - metabolism; p53 protein; Protein-Serine-Threonine Kinases - genetics; Protein-Serine-Threonine Kinases - metabolism; Receptors, Transforming Growth Factor beta - genetics; Receptors, Transforming Growth Factor beta - metabolism; Sucrose; Sucrose - pharmacology; Vertebrates: reproduction; Vitrification; Goat; Immature oocyte; Gene expression; Oocyte secreted factors; Vitrification; Biology; Meiosis; Germinal cell; Cryobiology; Vertebrata; Mammalia; Animal; Artiodactyla; Ungulata; Oocyte; Apoptosis; Biomedical engineering
• ISSN: 0011-2240; 1090-2392
• DOI: 10.1016/j.cryobiol.2012.01.005

The aim of the study was to evaluate meiotic maturation, and expression of genes coding for oocyte secreted factors (GDF9, BMP15, TGFBR1, and BPR2) and apoptosis (BCL2, BAX and P53) after vitrification of immature goat cumulus oocyte complexes (COCs) and in vitro maturation. COCs were vitrified in a solution containing ethylene glycol, dimethyl sulfoxide and sucrose using either a conventional straw (CS), open pulled straw (OPS), cryoloop (CL), hemistraw (HS) or cryotop (CT). Freshly collected COCs (Control), COCs exposed to vitrification and dilution solutions without cryopreservation (EC) and vitrified–warmed COCs were matured in vitro for 27h. The viability of vitrified–warmed COCs 2h post warming and in vitro maturation was similar for CL, HS and CT. The proportion of oocytes that extruded a 1st polar body and reached TI/MII was significantly higher with CT and HS followed by CL, OPS and CS. Gene expression of GDF9, BMP15, BMPR2, BAX and P53 were comparable to control levels for OPS, CL, HS and CT. The gene expression pattern in CS vitrified COCs was by contrast changed in that GDF9, BMP15, TGFBR1 and BAX were up regulated and BMPR2, BCL2 and P53 down regulated. In conclusion immature goat COCs vitrified using CT and HS showed that viability, maturation rates and expression of genes coding for oocyte secreted factors and apoptosis were similar to non-vitrified controls.