Comparisons among six strains of Tetrahymena by microcalorimetry

• Published in: Journal of thermal analysis and calorimetry Vol. 115; no. 3; pp. 2151 - 2158
• Main Authors: Chen, X. J., Feng, W. S., Yu, Y. H.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-03-2014; Springer
• Subjects: Analytical Chemistry; Calorimetry; Chemistry; Chemistry and Materials Science; Clustering; Enthalpy; Inorganic Chemistry; Maximum power; Measurement Science and Instrumentation; Physical Chemistry; Polymer Sciences; Rate constants; Spacers; Strain; Thermal analysis; Microcalorimetry; ITS1; Metabolic characteristics; Power–time curves
• ISSN: 1388-6150; 1588-2926; 1572-8943
• DOI: 10.1007/s10973-013-3385-8

The typical power–time curves of six strains of
Tetrahymena
were determined by microcalorimetry at 28 °C. Metabolic characteristics were obtained including metabolic rate constant (
r
), heat output in the log phase (
Q
log
), total heat production (
Q
T
), peak time, and measured and potential maximum power output (
P
m
and
P
max
). The power–time curves and metabolic characteristics show the differences among
Tetrahymena
strains. Euclidean distances and a clustering analysis diagram were obtained by metabolic properties. The smaller distances are between the four strains of
Tetrahymena thermophila
and the largest distance exists between
Tetrahymena pyriformis
GL and
Tetrahymena pigmentosa
HG2. These results are consistent with those obtained by first internal transcriptional spacer sequences. Therefore, the power–time curves and metabolic properties can be noticeable characteristics in distinguishing the different strains of
Tetrahymena
. And, the heat output in the log phase (
Q
log
) is probably the most specific characteristic in distinguishing the different
Tetrahymena
strains and studying their relationships.