Yeast β-galactosidase in solid-state fermentations

• Published in: Enzyme and microbial technology Vol. 19; no. 1; pp. 39 - 44
• Main Authors: Becerra, M., González Siso, M.I.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 1996; Elsevier Science
• Subjects: Biological and medical sciences; Biotechnology; Enzyme engineering; Fermentation; Fundamental and applied biological sciences. Psychology; Kluyveromyces lactis; Methods. Procedures. Technologies; milk whey; Production of selected enzymes; Solid-state fermentations; β-galactosidase; Kluyveromyces lactis; β-galactosidase; milk whey; Solid-state fermentations; Kluyveromyces marxianus var. lactis; Yeast; Enzyme; Whey; Optimization; Fungi; Glycosidases; Production; Hydrolases; Solid substrate fermentation; Ascomycetes; O-Glycosidases; β-Galactosidase; Thallophyta
• ISSN: 0141-0229; 1879-0909
• DOI: 10.1016/0141-0229(95)00180-8

A simple and economical system is proposed for producing β-galactosidase with the yeast
Kluyveromyces lactis in solid-state fermentations on corn grits or wheat bran moistened with deproteinized milk whey. By means of a full-factorial design, we studied some conditions that improve this production. Results of liquid- and solid-state cultures were compared with the latter proving to be more effective. We have demonstrated that changing from liquid- to solid-state cultures does not promote β-galactosidase secretion by
Kluyveromyces lactis.