Prevalence of multidrug-resistant Staphylococcus aureus in diabetics clinical samples

• Published in: World journal of microbiology & biotechnology Vol. 26; no. 1; pp. 171 - 176
• Main Authors: Raju, S, Oli, Ajay Kumar, Patil, S. A, Kelmani Chandrakanth, R
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 2010; Springer Netherlands; Springer; Springer Nature B.V
• Subjects: Abscesses; Antibiotic resistance; Antibiotics; Antimicrobial agents; Applied Microbiology; Bacterial infections; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Diabetes; Drug resistance; Environmental Engineering/Biotechnology; Enzymes; Fundamental and applied biological sciences. Psychology; Genes; Genomes; Life Sciences; Microbiology; Multidrug resistant organisms; Original Paper; Pathogens; Patients; Penicillin; Staphylococcus aureus; Staphylococcus infections; Studies; Urinary tract diseases; Urinary tract infections; Urogenital system; India; and; Methicillin resistant; Aminoglycoside modifying enzymes; Prevalence; Enzyme; Diabetes mellitus; Sample; Meticillin; Aminoglycoside; Multiple resistance; Bacteria; Micrococcales; Methicillin resistant Staphylococcus aureus; Micrococcaceae; mecA and femA; Staphylococcus aureus
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-009-0157-5

Antibiotic resistance in 40 Staphylococcus aureus clinical isolates from 110 diabetic patients (36%) was evaluated. Of these, 32 (80%) of the isolates showed multidrug-resistance to more than eight antibiotics and 35% isolates were found to be methicillin resistant S. aureus (MRSA). All 40 S. aureus strains (100%) screened from diabetic clinical specimens were resistant to penicillin, 63% to ampicillin, 55% to streptomycin, 50% to tetracycline and 50% to gentamicin. Where as low resistance rate was observed to ciprofloxacin (20%) and rifampicin (8%). In contrast, all (100%) S. aureus strains recorded susceptibility to teicoplanin, which was followed by vancomycin (95%). Genotypical examination revealed that 80% of the aminoglycoside resistant S. aureus (ARSA) have aminoglycoside modifying enzyme (AME) coding genes; however, 20% of ARSA which showed non-AME mediated (adaptive) aminoglycoside resistance lacked these genes in their genome. In contrast all MRSA isolates possessed mecA, femA genetic determinants in their genome.