Detection and Analysis of Chimeric Tertiary Structures by Backbone Thioester Exchange: Packing of an α Helix against an α/β-Peptide Helix

Backbone thioester exchange is used to explore a fundamental type of protein–foldamer packing motif, the association of an α helix and an α/β‐peptide foldameric helix, which is analogous to an antiparallel coiled‐coil tertiary structure in a pure α‐residue backbone. Side‐chain packing preferences at...

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Bibliographic Details
Published in:	Angewandte Chemie (International ed.) Vol. 49; no. 2; pp. 368 - 371
Main Authors:	Price, Joshua L, Hadley, Erik B, Steinkruger, Jay D, Gellman, Samuel H
Format:	Journal Article
Language:	English
Published:	Weinheim Wiley-VCH Verlag 01-01-2010 WILEY-VCH Verlag WILEY‐VCH Verlag
Subjects:	Amino Acids - chemistry biophysics Chromatography, High Pressure Liquid Computational Biology Esters - chemistry foldamers Helix-Loop-Helix Motifs Hydrogen-Ion Concentration Mutant Chimeric Proteins - chemical synthesis Mutant Chimeric Proteins - chemistry peptides Peptides - chemical synthesis Peptides - chemistry Protein Conformation Protein Folding Protein Structure, Secondary Structure-Activity Relationship Sulfhydryl Compounds - chemistry Thermodynamics thioesters Ultracentrifugation
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Summary:	Backbone thioester exchange is used to explore a fundamental type of protein–foldamer packing motif, the association of an α helix and an α/β‐peptide foldameric helix, which is analogous to an antiparallel coiled‐coil tertiary structure in a pure α‐residue backbone. Side‐chain packing preferences at this chimeric tertiary interface are comparable to those that determine pairing propensities among antiparallel α helices.
Bibliography:	http://dx.doi.org/10.1002/anie.200904714 ark:/67375/WNG-94LWD1D1-5 NIH - No. GM-61238; No. T32 GM008505 istex:D9DC7F15351AB6BEDCB07388E143E6090CA509B2 This research was supported by the NIH Grant GM-61238. J.L.P. was supported in part by an NIH Chemical Biology Training Grant (T32 GM008505). We thank Dr. Darrell McCaslin for assistance with AU experiments, and Peptech for providing β3-amino acids at a discounted price. ArticleID:ANIE200904714 This research was supported by the NIH Grant GM‐61238. J.L.P. was supported in part by an NIH Chemical Biology Training Grant (T32 GM008505). We thank Dr. Darrell McCaslin for assistance with AU experiments, and Peptech for providing β 3 These authors contributed equally. amino acids at a discounted price. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.200904714