Antibacterial and Osteoconductive Effects of Chitosan/Polyethylene Oxide (PEO)/Bioactive Glass Nanofibers for Orthopedic Applications

• Published in: Applied sciences Vol. 10; no. 7; p. 2360
• Main Authors: Boschetto, Francesco, Ngoc Doan, Hoan, Phong Vo, Phu, Zanocco, Matteo, Zhu, Wenliang, Sakai, Wataru, Adachi, Tetsuya, Ohgitani, Eriko, Tsutsumi, Naoto, Mazda, Osam, Kinashi, Kenji, Marin, Elia, Pezzotti, Giuseppe
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01-04-2020
• Subjects: Aluminum; antibacterial; Antimicrobial agents; Biocompatibility; Bioglass; Biological activity; Biomedical materials; Bone matrix; Chitosan; Coatings; composite; Contact angle; Dental materials; Dental restorative materials; Extracellular matrix; Fluorescence; Fluorescence microscopy; Fourier transforms; In vitro methods and tests; Microscopy; Mineralization; Nanofiber; Nanofibers; Orthopedics; Osteoblasts; Osteocalcin; Osteoconduction; osteoconductive; Osteopontin; Osteosarcoma; PEO; Physiochemistry; Polyethylene; Polyethylene oxide; Polymers; Scanning electron microscopy; Software; Spectrum analysis; Substrates; Titanium; Titanium alloys; Viability; United States > US; Japan
• ISSN: 2076-3417; 2076-3417
• DOI: 10.3390/app10072360

This study investigated the efficiency of chitosan/polyethylene oxide (PEO)-based nanofibers with incorporated bioactive glass particles as a coating for titanium alloy, in order to improve the bacteriostatic behavior and, concurrently, promote the production of mineralized tissue. Nanofibers with and without bioglass powder were fabricated by electrospinning technique and characterized using several microscopic and spectroscopic techniques in order to study their morphological and physiochemical properties. Subsequently, the substrates were tested in vitro against Staphylococcus epidermidis and SaOS-2 human osteosarcoma cell line. After in vitro testing, viability and CFU counting assays combined with fluorescence microscopy showed a clear decrease in bacterial growth on all substrates with increasing time. However, this trend was stronger for substrates coated with nanofibers. Formation of mineralized matrix upon exposure to osteoblasts was confirmed by means of SEM/EDX and the content/distribution of osteocalcin and osteopontin estimated by fluorescence microscopy. Incorporation of bioglass promoted biomineralization and stimulated osteoblasts to produce a higher amount of bone extracellular matrix. The present results suggest that a chitosan/PEO/bioactive glass nanofiber composite applied as coating on titanium alloys could concurrently improve antibacterial and osteoconductive properties and could be a potential candidate for dental and orthopedic applications.