Stacking of Pup1 QTL for low soil phosphorus tolerance and bacterial blight resistance genes in the background of APMS6B, the maintainer line of rice hybrid DRRH-3

• Published in: Euphytica Vol. 218; no. 4
• Main Authors: Madhusudan, N., Beulah, P., Jaldhani, V., Nagaraju, P., Manasa, Y., Sundaram, R. M., Laha, G. S., Anantha, M. S., Barbadikar, Kalyani M., Gireesh, C., HariPrasad, A. S., Madhav, M. Sheshu, Gobinath, R., Yugandhar, A., Kemparaju, K. B., Neeraja, C. N., Brajendra, P., Tuti, M. D., Kumar, R. Mahender, Krishna, K. V. Radha, Senguttuvel, P.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-04-2022; Springer; Springer Nature B.V
• Subjects: Agricultural production; Bacteria; Bacterial genetics; Bacterial infections; Biomedical and Life Sciences; Biotechnology; Blight; Crop diseases; Crop yield; Disease resistance; Fertilizers; Genes; Genetic research; Genomes; Heterosis; Hybrids; Inoculum; Kinases; Leaf blight; Leaves; Life Sciences; Markers; Oryza sativa; Phosphorus; Phosphorus content; Phosphorus pentoxide; Plant Genetics and Genomics; Plant growth; Plant Pathology; Plant Physiology; Plant Sciences; Quantitative genetics; Quantitative trait loci; Rainy season; Rice; Soil microbiology; Soil microorganisms; Soils; Starvation; Xanthomonas oryzae; Hybrids; Low phosphorus tolerance; APMS6B; MABB; Maintainer line; Rice; Bacterial blight
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1007/s10681-022-02987-0

Phosphorus (P) is one of the macronutrients essential for plant growth and development. Rice (
Oryza sativa
L.) is sensitive to P starvation and its deficiency influences many key plant functions, resulting in crop yield penalties. Although the hybrid rice segment is well-known for its yield heterosis, P deficiency and bacterial leaf blight diseases are evident limitations. APMS6B, the female parent of DRRH-3 is susceptible to low P and bacterial blight disease. In the present study, the improvement of APMS6B to P starvation and resistance to bacterial leaf blight (BB) was carried out using the marker-assisted backcross breeding approach. Kasalath (+
Pup1
QTL) was used as a donor, and a promising IL (ATR 594-1) at BC
1
F
4
generation was identified with 81.15% of recurrent parent genome recovery. Concurrently, this IL was intercrossed with GU-2 (+
Xa21
and
Xa38
). Intercross F
1
s (ICF
1
) hybridity was confirmed through foreground selection having maximum RPGR (88.29%) and was selfed to produce ICF
2
. The resultant progenies were phenotyped for BB using
Xoo
inoculum (IX-020), simultaneously genotyped with gene-specific functional SSR markers for
Xa21
and
Xa38
. The identified BB-resistant plants were subjected to foreground selection for
Pup1
. Four promising ICF
3
plants (BP-10-1, BP-10-3, BP-10-5, and BP-10-15 with
Xa21, Xa38, and Pup1
) along with parents and checks were screened both in low P plot (< 2 kg P
2
O
5
 ha
−1
) as well as in normal plot (> 25 kg P
2
O
5
 ha
−1
) during
dry
and
wet
seasons 2018. The field evaluation identified four promising intercrossed lines with better root growth in the primary root length of extracted zone and root volume. In addition, fewer reductions in grain yield (39.10%) under P starvation and less susceptibility indices values (< 1) for BB were observed. These lines may be exploited in the CMS conversion and development of climate-resilient, biotic and abiotic stress-tolerant rice hybrids.