Pseudomonas aeruginosa keratitis in leukopenic rabbits

• Published in: Current eye research Vol. 12; no. 5; p. 461
• Main Authors: Hobden, J A, Engel, L S, Callegan, M C, Hill, J M, Gebhardt, B M, O'Callaghan, R J
• Format: Journal Article
• Language: English
• Published: England 1993
• Subjects: Animals; Colony Count, Microbial; Corneal Ulcer - microbiology; Corneal Ulcer - pathology; Disease Models, Animal; Eye Infections, Bacterial - microbiology; Immunocompromised Host; Leukopenia - complications; Neutrophils - immunology; Pseudomonas Infections - microbiology; Rabbits
• ISSN: 0271-3683
• DOI: 10.3109/02713689309024628

To study the role of the host inflammatory response in Pseudomonas aeruginosa keratitis, rabbits were made leukopenic with intravenous injections of cyclophosphamide and dexamethasone. Twenty-four hr later, keratitis was initiated in all rabbits with an intrastromal injection of 1,000 log phase P. aeruginosa strain 27853. Slit lamp examination of eyes showed that leukopenic rabbits had significantly less (P < 0.0001) ocular pathology at 16, 22, and 27 hr postinfection. The number of viable bacteria recovered from corneas of leukopenic rabbits was the same as the number recovered from nonleukopenic rabbits (P = 0.95). These results suggest that the host inflammatory response significantly contributes to the overall ocular pathology associated with P. aeruginosa keratitis, but does not influence the survival of the infecting organism in the cornea at the height of the infection.