Seven days of exercise increase GLUT-4 protein content in human skeletal muscle

• Published in: Journal of applied physiology (1985) Vol. 79; no. 6; p. 1936
• Main Authors: Houmard, J A, Hickey, M S, Tyndall, G L, Gavigan, K E, Dohm, G L
• Format: Journal Article
• Language: English
• Published: United States 01-12-1995
• Subjects: Blotting, Western; Exercise - physiology; Humans; Male; Monosaccharide Transport Proteins - metabolism; Muscle, Skeletal - metabolism; Time Factors
• ISSN: 8750-7587
• DOI: 10.1152/jappl.1995.79.6.1936

Insulin-responsive glucose transporter (GLUT-4) content increases by 1.8-fold in skeletal muscle with 14 wk of exercise training [Houmard et al. Am. J. Physiol. 264 (Endocrinol. Metab. 27): E896-E901, 1993]. The purpose of this study was to determine whether more short-term training (7 days) increases GLUT-4 protein content in human skeletal muscle. Seven sedentary men [25.0 +/- 1.1 (SE) yr, 44.1 +/- 2.2 ml.kg-1.min-1 maximal O2 uptake, 14.9 +/- 2.1% body fat] were examined before and after 7 days of cycle ergometer training (1 h/day, 76 +/- 2% maximal heart rate). Needle biopsy samples from the vastus lateralis were used to determine GLUT-4 protein content. Muscle GLUT-4 increased (P < 0.05) by an average of 2.8 +/- 0.5-fold with 7 days of training. GLUT-4 content in skeletal muscle thus increases substantially with short-term exercise training.