High expressions of the cytoglobin and PGC-1α genes during the tissue regeneration of house gecko (Hemidactylus platyurus) tails

• Published in: BMC developmental biology Vol. 20; no. 1; p. 11
• Main Authors: Novianti, Titta, Juniantito, Vetnizah, Jusuf, Ahmad Aulia, Arida, Evy Ayu, Sadikin, Mohamad, Jusman, Sri Widia A
• Format: Journal Article
• Language: English
• Published: England BioMed Central 11-05-2020
• Subjects: Animal models; Biosynthesis; Cartilage; Cell growth; Energy; Fibroblasts; Gene expression; Hemidactylus; Histology; Hypoxia; Metabolism; Mitochondria; Morphology; Oxidative stress; Oxygen; Proteins; Stem cells; Tails; Tissue engineering; Wound healing; Cytoglobin; House gecko; PGC-1α; Mitochondrial biogenesis; Tissue regeneration
• ISSN: 1471-213X; 1471-213X
• DOI: 10.1186/s12861-020-00214-4

The tissue regeneration process requires high oxygen and energy levels. Cytoglobin (Cygb) is a member of the globin family, which has the ability to bind oxygen, plays a role in dealing with oxidative stress, and carries oxygen into the mitochondria. Energy production for tissue regeneration is associated with mitochondria-especially mitochondrial biogenesis. The peroxisome proliferator-activated receptor-gamma coactivator (PGC)-1alpha protein helps to regulate mitochondrial biogenesis. House geckos (Hemidactylus platyurus) are reptiles that have the ability to regenerate the tissue in their tails. House geckos were selected as the animal models for this study in order to analyze the association of Cygb with oxygen supply and the association of PGC-1α with energy production for tissue regeneration.
The growth of house gecko tails showed a slow growth at the wound healing phase, then followed by a fast growth after wound healing phase of the regeneration process. While Cygb mRNA expression reached its peak at the wound healing phase and slowly decreased until the end of the observation. PGC-1α mRNA was expressed and reached its peak earlier than Cygb.
The expressions of both the Cygb and PGC-1α genes were relatively high compared to the control group. We therefore suggest that Cygb and PGC-1α play an important role during the tissue regeneration process.