Organic–inorganic matrix for electrochemical immunoassay: Detection of human IgG based on ZnO/chitosan composite

• Published in: Talanta (Oxford) Vol. 69; no. 3; pp. 686 - 690
• Main Authors: Wang, Zhijie, Yang, Yunhui, Li, Jishan, Gong, jilai, Shen, Guoli, Yu, Ruqin
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15-05-2006
• Subjects: Electrochemical immunoassay; Human IgG; Organic–inorganic matrix; ZnO/chitosan composite; ZnO/chitosan composite; Electrochemical immunoassay; Organic–inorganic matrix; Human IgG
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2005.11.004

A new strategy to construct amperometric immunosensor for human IgG assay based on ZnO/chitosan composite as sensing platform has been described. This material, which combined the advantages of inorganic species, ZnO and organic polymer, chitosan, can maintain biological activity well. A sequential sandwich immunoassay format was performed on the ZnO/chitosan composite supported by glass carbon electrode (GCE) using goat-anti-human IgG antibody (IgG Ab) and human IgG as a model system. Amperometry was used to determine the amount of horse-radish peroxidase (HRP) fixed on the sensor surface, which was related to the content of the desired human IgG. Assay conditions that were optimized included the amount of labeled antibody, the incubation time and temperature, the pH of the substrate solution, etc. Using hydroquinone as a mediator, amperometric detection at −150
mV (versus SCE) resulted in a detection range 2.5–500
ng
mL
−1, with a detection limit of 1.2
ng
mL
−1. The simple manipulations of the construction of ZnO/chitosan composite, as well as low-cost and broad linear range, are the main features of the proposed immunosensing method.