Characterization of the quisqualate receptor linked to phosphoinositide hydrolysis in neurocortical cultures

• Published in: Journal of neurochemistry Vol. 54; no. 5; p. 1461
• Main Authors: Patel, J, Moore, W C, Thompson, C, Keith, R A, Salama, A I
• Format: Journal Article
• Language: English
• Published: England 01-05-1990
• Subjects: Amino Acids - antagonists & inhibitors; Amino Acids - pharmacology; Animals; Cells, Cultured; Cerebral Cortex - cytology; Cerebral Cortex - metabolism; Hydrolysis; Inositol Phosphates - metabolism; Neurons - metabolism; Phosphatidylinositols - metabolism; Receptors, Amino Acid; Receptors, AMPA; Receptors, Cell Surface - drug effects; Receptors, Neurotransmitter - metabolism
• ISSN: 0022-3042
• DOI: 10.1111/j.1471-4159.1990.tb01192.x

Activation of phosphoinositide metabolism is an early event in signal transduction for a number of neurotransmitters and hormones. In primary cultures of rat neurocortical cells, various excitatory amino acids stimulate inositol phosphate production with a rank order of potency of quisqualate greater than ibotenate greater than glutamate greater than kainate, N-methyl-D-aspartate greater than alpha-amino-3-hydroxyl-5-methyl-4-isoxazole propionate. This response to excitatory amino acids was insensitive to a variety of excitatory amino acid antagonists including 6-cyano-7-nitroquinoxaline-2,3-dione, 3-3(2-carboxypiperazine-4-yl)propyl-1-phosphonate, and 2-amino-4-phosphonobutyrate. The individual responses of quisqualate-, ibotenate-, and kainate-stimulated inositol phosphate production were not additive. These results suggest that phosphoinositide metabolism activated by excitatory amino acids is mediated by a unique quisqualate-preferring receptor that is not antagonized by known N-methyl-D-aspartate and non-N-methyl-D-aspartate antagonists, and is relatively insensitive to alpha-amino-3-hydroxyl-5-methyl-4-isoxazole propionate.