Mycoplasma hyorhinis infection levels in lungs of piglets with porcine reproductive and respiratory syndrome (PRRS)

• Published in: Journal of Veterinary Medical Science Vol. 58; no. 2; pp. 109 - 113
• Main Authors: Kobayashi, H. (National Inst. of Animal Health, Tsukuba, Ibaraki (Japan)), Morozumi, T, Miyamoto, C, Shimizu, M, Yamada, S, Ohashi, S, Kubo, M, Kimura, K, Mitani, K, Ito, N, Yamamoto, K
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01-02-1996; Japan Science and Technology Agency
• Subjects: Animals; Antibodies, Bacterial - analysis; Antibodies, Bacterial - immunology; Antibodies, Viral - blood; Arterivirus - immunology; Arterivirus - isolation & purification; Arterivirus Infections - complications; Arterivirus Infections - epidemiology; Arterivirus Infections - veterinary; Base Sequence; DNA Primers - chemistry; DNA, Bacterial - analysis; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Haemophilus - genetics; Haemophilus - immunology; Haemophilus - isolation & purification; LECHON; Lung - microbiology; Lung - virology; LUNGS; Molecular Sequence Data; MYCOPLASMA; Mycoplasma - genetics; Mycoplasma - immunology; Mycoplasma - isolation & purification; MYCOPLASMA HYORHINIS; Mycoplasma Infections - complications; Mycoplasma Infections - epidemiology; Mycoplasma Infections - veterinary; Palatine Tonsil - virology; Pasteurella - genetics; Pasteurella - immunology; Pasteurella - isolation & purification; PCR; PIGLETS; Polymerase Chain Reaction - veterinary; PORCELET; POUMON; PRRS; PULMONES; SDRP; SRRP; Swine; Swine Diseases - epidemiology; Swine Diseases - microbiology; Syndrome; VIROSE; VIROSES; VIROSIS
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.58.109

The infection levels of Mycoplasma hyorhinis, M.hyopneumoniae and M.hyosynoviae in the lung of piglets were examined in relation to porcine reproductive and respiratory syndrome (PRRS). These animals consisted of 43 PRRS piglets with PRRS, 2 piglets infected with PRRS virus but symptom-free, and 10 control piglets free of PRRS virus and its antibody. M.hyorhinis was isolated from 40 of the 43 PRRS piglets, from 1 of the 2 latent infected piglets and from 3 of the 10 control piglets. The number of M.hyorhinis isolated from the lungs of PRRS piglets was more than 105 CFU/g, but those isolated from the latent infected piglets and the control piglets were less than 103 CFU/g. In addition to this, Haemophilus parasuis and Pasteurella spp. were frequently isolated from the piglets with PRRS (51.2% and 25.6%, respectively). On the other hand, M.hyopneumoniae was isolated from only 4 of 55 piglets tested, and M.hyosynoviae was not isolated. M.hyorhinis was also detected directly in the lung emulsion samples from almost all the PRRS piglets using a polymerase chain reaction-based method.