Phenylpropanoid glycoside inhibition of pepsin, trypsin and α-chymotrypsin enzyme activity in Kudingcha leaves from Ligustrum purpurascens

• Published in: Food research international Vol. 54; no. 2; pp. 1376 - 1382
• Main Authors: Wu, Xuli, Wang, WenPu, Zhu, Tian, Liang, Ting, Lu, FangQi, He, Weiyi, Zhang, Haiping, Liu, Zhigang, He, ShaoHeng, Gao, Kaiping, He, Zhendan
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01-12-2013; Elsevier
• Subjects: Biological and medical sciences; Enzyme activity; Enzymes; Food industries; Foods; Fundamental and applied biological sciences. Psychology; Glycosides; ingredients; Inhibition; Inhibitor; Interaction; leaves; Ligustrum robustum subsp. chinense; Pepsin; Phenylpropanoid glycoside; Polarity; tea; Trypsin; α-Chymotrypsin; α-Chymotrypsin; Trypsin; Inhibitor; Pepsin; Phenylpropanoid glycoside; Interaction; Oleaceae; Serine endopeptidases; Enzyme; Plant leaf; Peptidases; Chymotrypsin; Plant part; Enzymatic activity; Dicotyledones; Angiospermae; Hydrolases; Spermatophyta; Glycoside; Inhibition; Ligustrum
• ISSN: 0963-9969; 1873-7145
• DOI: 10.1016/j.foodres.2013.10.020

Phenylpropanoid glycosides (PPGs) are important ingredients in Kudingcha bitter tea from Ligustrum purpurascens, possessing many beneficial properties. Here, we investigated the effect of PPGs on the activity of the digestive enzymes pepsin, trypsin and α-chymotrypsin. Furthermore, we explored the interactions between PPGs and digestive enzymes by a multi-spectroscopic method and docking studies. PPGs could inhibit digestive enzyme activities with a non-competitive pattern of inhibition. PPGs may have a binding effect on these digestive enzymes, changing the polarity and the structure of enzymes, which results in decreased enzyme activity. This description of the effects of PPG inactivation of pepsin, trypsin and α-chymotrypsin helps reveal the potential anti-nutritional property of PPGs.
•Kudingcha (bitter tea) has been used for health in China for a long time.•Phenylpropanoid glycosides are important ingredients in Kudingcha.•Phenylpropanoid glycosides have a binding effect on digestive enzymes.•Phenylpropanoid glycosides could inhibit digestive enzyme activities.