Application of genomic in situ hybridization for phylogenetic study between Mangifera indica L. and eight wild species of Mangifera

• Published in: Scientia horticulturae Vol. 110; no. 1; pp. 114 - 117
• Main Authors: Nishiyama, Kiyomi, Choi, Young A., Honsho, Chitose, Eiadthong, Wichan, Yonemori, Keizo
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 11-09-2006; Elsevier
• Subjects: Agronomy. Soil science and plant productions; amplified fragment length polymorphism; Biological and medical sciences; chromosomes; Fundamental and applied biological sciences. Psychology; genetic markers; Genetics and breeding of economic plants; genome; Genomic in situ hybridization (GISH); Mangifera; Mangifera indica; Mango ( Mangifera indica L.); mangoes; molecular systematics; nucleic acid hybridization; Phylogenetic relationship; phylogeny; plant genetic resources; plant taxonomy; wild plants; wild relatives; Phylogenetic relationship; Mango ( Mangifera indica L.); Mangifera; Genomic in situ hybridization (GISH); Horticulture; Phylogenetic tree; Mangifera indica; Phylogeny; Mango (Mangifera indica L.); Dicotyledones; Angiospermae; Fluorescence in situ hybridization; Wild plant; Spermatophyta; Fruit tree; Anacardiaceae
• ISSN: 0304-4238; 1879-1018
• DOI: 10.1016/j.scienta.2006.06.005

The phylogenetic relationship between mango (
Mangifera indica L.) and eight wild species of
Mangifera were analyzed by comparing signal intensity of genomic in situ hybridization (GISH) on somatic metaphase chromosomes of
M. indica, using labeled DNA of eight wild
Mangifera species. The eight wild species were divided into four groups based on intensity and number of hybridization signals on chromosomes of
M. indica in GISH analyses. The probe of
Mangifera sylvatica Roxb. gave the highest intensities on the chromosome of
M. indica, indicating a close relationship between
M. indica and
M. sylvatica. For the other species, classification of GISH was comparable to that of the phylogenetic analysis using AFLP markers, as previously reported (
Eiadthong et al., 2000). This suggested a possibility that GISH analysis can be effectively used in the classification of
Mangifera species.