Multipanel Approach including miRNAs, Inflammatory Markers, and Depressive Symptoms for Metabolic Dysfunction-Associated Steatotic Liver Disease Diagnosis during 2-Year Nutritional Intervention

• Published in: Nutrients Vol. 16; no. 11; p. 1547
• Main Authors: Tobaruela-Resola, Ana Luz, Riezu-Boj, José I, Milagro, Fermin I, Mogna-Pelaez, Paola, Herrero, José I, Elorz, Mariana, Benito-Boillos, Alberto, Tur, Josep A, Martínez, J Alfredo, Abete, Itziar, Zulet, M Angeles
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 21-05-2024; MDPI
• Subjects: Adult; Biomarkers; Biomarkers - blood; Biopsy; Body composition; Body fat; Body Mass Index; Carbohydrates; Cholesterol; circulating miRNA; Depression - blood; Depression - diagnosis; Depression - etiology; Depression, Mental; Diabetes; Diet; Exercise; Fatty Liver - blood; Fatty Liver - diagnosis; Fatty Liver - etiology; Female; Food; Glucose; High density lipoprotein; Humans; Inflammation; Inflammation - blood; inflammatory status; Insulin resistance; Intervention; Lifestyles; Lipids; Liver - metabolism; Liver diseases; Male; MASLD; Medical research; Medicine, Experimental; Metabolic syndrome; MicroRNA; MicroRNAs - blood; Middle Aged; NAFLD; Non-alcoholic Fatty Liver Disease - blood; Obesity; Obesity - complications; Proteins; Questionnaires; Triglycerides - blood; Type 2 diabetes; Ultrasonic imaging; Weight control; weight loss; Canada; Germany; Spain; United States > US; NAFLD; biomarkers; long-term follow-up; circulating miRNA; weight loss; fatty liver; depression; MASLD; dietary intervention; inflammatory status
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu16111547

Metabolic dysfunction-associated steatotic liver disease (MASLD), with a prevalence of 30% of adults globally, is considered a multifactorial disease. There is a lack of effective non-invasive methods for accurate diagnosis and monitoring. Therefore, this study aimed to explore associations between changes in circulating miRNA levels, inflammatory markers, and depressive symptoms with hepatic variables in MASLD subjects and their combined potential to predict the disease after following a dietary intervention. Biochemical markers, body composition, circulating miRNAs and hepatic and psychological status of 55 subjects with MASLD with obesity and overweight from the FLiO study were evaluated by undergoing a 6-, 12- and 24-month nutritional intervention. The highest accuracy values of combined panels to predict the disease were identified after 24 months. A combination panel that included changes in liver stiffness, high-density lipoprotein cholesterol (HDL-c), body mass index (BMI), depressive symptoms, and triglycerides (TG) yielded an AUC of 0.90. Another panel that included changes in hepatic fat content, total cholesterol (TC), miR15b-3p, TG, and depressive symptoms revealed an AUC of 0.89. These findings identify non-invasive biomarker panels including circulating miRNAs, inflammatory markers, depressive symptoms and other metabolic variables for predicting MASLD presence and emphasize the importance of precision nutrition in MASLD management and the sustained adherence to healthy lifestyle patterns.