Study of the effect of reducing conditions on the initial chlorophyll fluorescence rise in the green microalgae Chlamydomonas reinhardtii

• Published in: Photosynthesis research Vol. 114; no. 3; pp. 143 - 154
• Main Authors: Antal, T. K, Kolacheva, A, Maslakov, A, Riznichenko, G. Yu, Krendeleva, T. E, Rubin, A. B
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 01-03-2013; Springer Netherlands; Springer Nature B.V
• Subjects: Algae; Biochemistry; Biofuels; Biomedical and Life Sciences; Chlamydomonas reinhardtii; Chlamydomonas reinhardtii - metabolism; Chlorophyll; Chlorophyll - metabolism; Fluorescence; Kinetics; Life Sciences; Light; microalgae; Models, Biological; nutrient deficiencies; Oxidation-Reduction; Photosynthesis; photosystem II; Photosystem II Protein Complex - metabolism; Plant Genetics and Genomics; Plant Physiology; Plant Sciences; Plastoquinone - metabolism; Regular Paper; starvation; Sulfur - metabolism; Chlorophyll fluorescence; Sulfur deprivation; OEC integrity; PQ pool redox state
• ISSN: 0166-8595; 1573-5079
• DOI: 10.1007/s11120-012-9789-7

Incubation of Chlamydomonas reinhardtii cells under nutrient deficiency results in the faster initial rise in the light-induced chlorophyll fluorescence kinetic curve. We showed that short-term anaerobic incubation of algal cells altered initial fluorescence in a way similar to nutrient starvation, suggesting an important role of the plastoquinones redox state in the observed effect. Bi-component analysis of highly resolved initial fluorescence rise kinetics in sulfur- or oxygen-depleted C. reinhardtii cells suggested that one of the mechanisms underlying the observed phenomenon involves primary closure (photochemical inactivation via Qa reduction) of β-type PSII as compared to α-PSII. Moreover, results of modeling of the fluorescence curve brought us to the conclusion that accumulation of closed centers in α-PSII supercomplexes may also cause a faster initial fluorescence rise. The observed correlations between nutrient supply rate and initial fluorescence rise pattern in green algae can serve to characterize culture nutritional status in vivo.