pH-jump-induced phosphorylation of ADP in the cyanobacterium Anacystis nidulans

• Published in: FEBS letters Vol. 164; no. 1; pp. 116 - 120
• Main Authors: Muchl, Robert, Peschek, Günter A.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 28-11-1983; Elsevier
• Subjects: ADP; Anacystis nidulans; ATP; ATP synthesis; bioenergetics; Biological and medical sciences; biosynthesis; Cell structures and functions; Chloroplast, photosynthetic membrane and photosynthesis; Cytoplasmic membrane; cytoplasmic membranes; Freshwater; Fundamental and applied biological sciences. Psychology; Molecular and cellular biology; pH gradient; phosphorylation; Protonmotive force; Reversible H +-ATPase; ATP synthesis; Cytoplasmic membrane; pH gradient; Reversible H +-ATPase; Protonmotive force; Anacystis nidulans; Photophosphorylation; Photosystem; PH gradient; Cyanobacteria
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(83)80031-3

The low ATP levels in dark anaerobic cells of the cyanobacterium
Anacystis nidulans more than doubled within 5 s after rapid addition of HCl shifting external pH from 9.0 to 4.5. Steady-state levels of ATP and intracellular pH remained constant at 0.95 ± 0.15 nmol/mg dry weight and 6.9 ± 0.3, respectively. ΔpH-induced ATP synthesis was inhibited by dicyclohexylcarbodiimide and carbonyl cyanide
m-chlorophenylhydrazone but not by carbon monoxide. According to our results the cytoplasmic membrane of
A. nidulans has to be regarded as an energy-transducing membrane bioenergetically similar to the thylakoid membrane.