Preparation, characterization, in-vitro drug release and cellular uptake of poly(caprolactone) grafted dextran copolymeric nanoparticles loaded with anticancer drug

• Published in: Journal of biomedical materials research. Part A Vol. 90A; no. 4; pp. 1128 - 1136
• Main Authors: Prabu, P., Chaudhari, Atul A., Dharmaraj, N., Khil, M. S., Park, S. Y., Kim, H. Y.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 15-09-2009
• Subjects: Antineoplastic Agents - administration & dosage; Antineoplastic Agents - pharmacokinetics; biodegradable polymers; Breast Neoplasms - drug therapy; cancer chemotherapy; Cell Line, Tumor; Cell Survival - drug effects; Dextrans; Drug Carriers - chemistry; Drug Carriers - pharmacokinetics; drug release; Female; Humans; nanoparticles; Nanoparticles - chemistry; Nanoparticles - therapeutic use; Polyesters; polymeric drug carriers; Vinblastine - administration & dosage; Vinblastine - pharmacokinetics
• ISSN: 1549-3296; 1552-4965
• DOI: 10.1002/jbm.a.32163

Biodegradable and biocompatible polymers that are engineered to nanostructures play a key role in providing solution for sustained chemotherapy. This study is focused on preparation, drug encapsulation efficiency, in‐vitro drug release, in‐vitro cellular uptake and cell viability of poly(caprolactone) grafted dextran (PGD) nanoparticles (NPs) formulation containing vinblastine as the anticancer drug. Drug‐loaded PGD NPs were prepared by a modified oil/water emulsion method and characterized by laser light scattering, atomic force microscopy (AFM), and zeta potential. The drug encapsulation efficiency was determined spectrophotometrically and in‐vitro drug release was estimated using dialysis bag. Breast cancer cell line (MCF‐7) was used to image and measure the cellular uptake of fluorescent PGD NPs. Cancer cell viability was assessed by treating MCF‐7 cells with vinblastine‐loaded PGD NPs by crystal violet staining method. Result showed that the vinblastine‐loaded PGD NPs were superior in properties such as drug encapsulation efficiency, the cellular uptake and the cancer cell mortality. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009