Assessment of Cytochrome c and Chlorophyll a as Natural Redox Mediators for Enzymatic Biofuel Cells Powered by Glucose

• Published in: Energies (Basel) Vol. 15; no. 18; p. 6838
• Main Authors: Samukaite Bubniene, Urte, Zukauskas, Sarunas, Ratautaite, Vilma, Vilkiene, Monika, Mockeviciene, Ieva, Liustrovaite, Viktorija, Drobysh, Maryia, Lisauskas, Aurimas, Ramanavicius, Simonas, Ramanavicius, Arunas
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01-09-2022
• Subjects: Algae; Biochemical fuel cells; Biodiesel fuels; Biofuels; Biomass energy; Biosensors; Carbon; Charge transfer; Chemical properties; Chlorella; Chlorella vulgaris; Chlorophyll; Chlorophyll a; Coronaviruses; COVID-19; Cytochrome; Cytochrome c; Dextrose; Electricity; Electrochemistry; Electrodes; Electron transfer; Enzymes; Evaluation; Fuel cells; Glucose; Glucose oxidase; Heparin; Medical equipment; Medical research; microbial-based biofuel cell; Nanoparticles; Open circuit voltage; Oxidation; Proteins; redox mediators; Severe acute respiratory syndrome coronavirus 2; Testing; the supernatant of ultrasonically disrupted Chlorella vulgaris cells; Lithuania
• ISSN: 1996-1073; 1996-1073
• DOI: 10.3390/en15186838

The development of new high-power biofuel cells has been limited in the past by slow or indirect charge transfer. In this study, enzymatic biofuel cell (EBFC) systems were explored with different materials used to evaluate their applicability as redox mediators. Redox mediators of different natures have been selected for this research. Cytochrome c, Chlorophyll a, and supernatant of ultrasonically disrupted algae Chlorella vulgaris cells were examined as potential redox mediators. The effect of heparin on the EBFC was also evaluated under the same analytical conditions. The measurements of open circuit potential (OCP) and the evaluation of the current response in two modes of measurement were performed (i) during potential cycling in cyclic voltammetry measurements or (ii) at the constant potential value in chronoamperometry, and were applied for the evaluation of EBFC. Cytochrome c, Chlorophyll a, and the supernatant of ultrasonically disrupted algae Chlorella vulgaris cells-based redox mediators were efficient in the glucose oxidase (GOx) based EBFC. Electron transfer from GOx to the electrode was facilitated through the redox mediators adsorbed on the electrode. Electrodes modified with Chlorophyll a- and Cytochrome c-based redox mediators were suitable for the development of glucose biosensors. This was demonstrated by increasing the glucose concentration within 0 mM–100 mM in the system, the current density increased, and the system reached equilibrium rather faster regarding the electrochemical reaction. The power density is an important feature in revealing the action of biofuel cells. The highest power values were generated by the systems based on the application of redox-mediated Chlorophyll a and the supernatant of ultrasonically disrupted Chlorella vulgaris cells. The surface power density was about 2.5–4.0 µW/cm2. Control of a study was performed with a polished graphite electrode and the maximum surface power density was 0.02471 µW/cm2.