Adjuvant effect of polysaccharide from fruits of Physalis alkekengi L. in DNA vaccine against systemic candidiasis

• Published in: Carbohydrate polymers Vol. 109; pp. 77 - 84
• Main Authors: Yang, Huimin, Han, Shuying, Zhao, Danyang, Wang, Guiyun
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 30-08-2014; Elsevier
• Subjects: Adjuvant; Adjuvants, Immunologic - administration & dosage; Animals; Antibodies, Fungal - blood; Applied sciences; Candida albicans; Candida albicans - immunology; Candidiasis - blood; Candidiasis - immunology; Candidiasis - prevention & control; Cytokines - blood; DNA vaccine; Drug Evaluation, Preclinical; Exact sciences and technology; Fruit - chemistry; Fungal Proteins - genetics; Fungal Proteins - immunology; Fungemia - prevention & control; HSP90 Heat-Shock Proteins - genetics; HSP90 Heat-Shock Proteins - immunology; Kidney - microbiology; Male; Mice, Inbred ICR; Natural polymers; Physalis - chemistry; Physalis alkekengi; Physalis alkekengi L. polysaccharide; Physicochemistry of polymers; Plant Extracts - administration & dosage; Polysaccharides - administration & dosage; Starch and polysaccharides; Vaccination; Vaccines, DNA - administration & dosage; Candida albicans; DNA vaccine; HSP90; CFU; rP-HSP90C; Physalis alkekengi L. polysaccharide; PPSB; Adjuvant; pD-HSP90C; Immunization; Fruit; Rodentia; Experimental study; Genetic vaccine; Intramuscular administration; Biological activity; Fungi; Infection; In vivo; Vertebrata; Mammalia; Mouse; Animal; Immunological adjuvant; Plant origin; Oside polymer; Fungi Imperfecti
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2014.03.054

•DNA vaccine rD-HSP90C evoked production of specific antibody against rP-HSP90C.•PPBS significantly enhanced specific antibody titers IgG, IgG1and IgG2b.•PPBS significantly promoted production of IL-2 and IL-4 in sera of mice.•PPSB significantly reduced the numbers of CFU in the kidneys of mice.•PPBS significantly augmented survival rate of mice.
Adjuvant effect mediated by polysaccharide (PPSB) isolated from the fruits of Physalis alkekengi L. in DNA vaccine was evaluated in mice. Recombinant plasmid containing epitope C (LKVIRK) from heat shock protein 90 (HSP90) of Candida albicans (C. albican) was used as DNA vaccine (pD-HSP90C). The results indicated that PPSB significantly enhanced specific antibody titers IgG, IgG1, IgG2b, and concentration of IL-2 and IL-4 in sera of mice immunized with pD-HSP90C (p<0.05). More importantly, it was found that the mice immunized with pD-HSP90C/PPSB not only had fewer CFU (colony forming unites) in the kidneys than mice immunized with pD-HSP90C, but also a statistically significant higher survival rate over PBS-injected group (p<0.05) when the immunized mice were challenged with living C. albican cells. However, no statistically significant difference in survival rate was observed between pD-HSP90C-immunized group and PBS-injected group. Therefore, PPSB can be considered as a promising adjuvant eliciting both Th1 and Th2 responses to enhance the efficacy of DNA vaccines.