Nanostructured materials for magnetic biosensing

• Published in: Biochimica et biophysica acta. General subjects Vol. 1861; no. 6; pp. 1494 - 1506
• Main Authors: Kurlyandskaya, Galina V., Portnov, Dmitriy S., Beketov, Igor V., Larrañaga, Aitor, Safronov, Alexander P., Orue, Iñaki, Medvedev, Anatoly I., Chlenova, Anna A., Sanchez-Ilarduya, Maria B., Martinez-Amesti, Ana, Svalov, Andrey V.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-06-2017
• Subjects: Biosensing Techniques; Colloids; Ferric Compounds - chemistry; Ferrogels; Gels; Giant magnetoimpedance; Iron oxide nanoparticles; Laser target evaporation; Magnetic biosensing; Magnetics - methods; Magnetite Nanoparticles - chemistry; Magnetite Nanoparticles - ultrastructure; Molecular Structure; Nanotechnology - methods; Particle Size; Spark discharge; Structure-Activity Relationship; Surface Properties; Magnetic biosensing; Laser target evaporation; Spark discharge; Iron oxide nanoparticles; Giant magnetoimpedance; Ferrogels
• ISSN: 0304-4165; 1872-8006
• DOI: 10.1016/j.bbagen.2016.12.003

Magnetic nanoparticles (MNPs) are at the leading edge of the field of biomedical applications and magnetic biosensing.
MNPs were fabricated by electrophysical methods of the laser target evaporation (LTE) and spark discharge with electrodynamic acceleration of plasma jumpers (SD). Synthesis of polyacrylamide hydrogel was done in the presence of Fe2O3 MNPs in different concentrations obtained by LTE. [FeNi/Ti]3/Cu/[Ti/FeNi]3/Ti multilayers for giant magnetoimpedance (GMI) based sensitive elements were prepared by rf-sputtering for testing a biosensor prototype.
Iron oxide MNPs, ferrofluids, ferrofluids contacting with biological systems, synthetic ferrogels mimicking natural tissues – are the steps of the discussed in this work development of bionanomaterials. Thorough the structural and magnetic studies of a multilayered sensitive element, MNPs and ferrogels insure the complete characterization of biosensor prototype. The GMI responses were carefully evaluated in initial state and in the presence of ferrogel with known concentration of MNPs. SD MNPs had the smallest 5–8nm size. This nanomaterial was characterized by large internal strains of the order of 25×10−3, which can play an important role for the interaction with different biosystems.
Iron oxide MNPs were fabricated by LTE and SD methods. SD MNPs had the smallest 5–8nm size and large internal strains of the order of 25×10−3. Designed GMI biosensor prototype allowed precise evaluation of the stray field of the MNPs present in the ferrogel by evaluating the systematic changes of the GMI in a 20–400MHz frequency range.
This work summarizes recent developments in the field of nanomaterials potentially applicable in magnetic biosensing.
•Magnetic nanoparticles (MNPs) fabricated by the laser target evaporation (LTE) and spark discharge (SD) techniques.•Electrostatically and sterically stabilized ferrofluids and acrilamid ferrogels were prepared on the LTE MNPs basis.•[FeNi/Ti]3/Cu/[Ti/FeNi]3/Ti multilayers for giant magnetoimpedance (GMI) sensitive elements were prepared by rf-sputtering.•GMI biosensor prototype was successfully tested in the regime of detection of MNPs in ferrogel.•Iron oxide MNPs fabricated by spark discharge insure the smallest size of 5 to 8nm.•SD MNPs are characterized by large internal strains of the order of 25×−3.