Analysis of leptin signalling in hematopoietic cells using an adapted MAPPIT strategy

• Published in: FEBS letters Vol. 580; no. 13; pp. 3301 - 3307
• Main Authors: Montoye, T., Piessevaux, J., Lavens, D., Wauman, J., Catteeuw, D., Vandekerckhove, J., Lemmens, I., Tavernier, J.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 29-05-2006
• Subjects: Amino Acid Motifs; Animals; Cells, Cultured; CIS; cytokine inducible SH2 containing protein; Hematopoietic Stem Cells - metabolism; Humans; Leptin - metabolism; Leptin receptor; MAPPIT; Mice; phospholipase C gamma; PLCγ; Rats; Receptors, Cell Surface - metabolism; Receptors, Leptin; signal transducer and activator of transcription; Signal Transduction; SOCS; STAT; STAT5 Transcription Factor - genetics; STAT5 Transcription Factor - metabolism; Suppressor of Cytokine Signaling Proteins - genetics; Suppressor of Cytokine Signaling Proteins - metabolism; suppressor of cytokine signalling; Two-Hybrid System Techniques; MAPPIT; Signal transduction; STAT; Leptin receptor; LR; PLCγ; SOCS; CIS
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2006.04.094

The adipocyte-secreted hormone leptin participates in the regulation of hematopoiesis and enhances proliferation of hematopoietic cells. We used an adaptation of the MAPPIT mammalian two-hybrid method to study leptin signalling in a hematopoietic setting. We confirmed the known interactions of suppressor of cytokine signalling 3 (SOCS3) and STAT5 with the Y985 and Y1077 motifs of the leptin receptor, respectively. We also provide evidence for novel interactions at the Y1077 motif, including phospholipase C gamma and several members of the SOCS protein family, further underscoring the important role of the Y1077 motif in leptin signalling.