Bacillus persepolensis sp. nov., a moderately halophilic bacterium from a hypersaline lake

• Published in: International journal of systematic and evolutionary microbiology Vol. 59; no. 9; pp. 2352 - 2358
• Main Authors: Amoozegar, M. A, Sanchez-Porro, C, Rohban, R, Hajighasemi, M, Ventosa, A
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01-09-2009; Society for General Microbiology
• Subjects: Aerobiosis; Bacillus; Bacillus - classification; Bacillus - genetics; Bacillus - isolation & purification; Bacillus - physiology; Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; Cluster Analysis; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fatty Acids - analysis; Firmicutes; Freshwater; Fundamental and applied biological sciences. Psychology; Hydrogen-Ion Concentration; Iran; Locomotion; Microbiology; Molecular Sequence Data; Peptidoglycan - analysis; Phospholipids - analysis; Phylogeny; Quinones - analysis; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Sodium Chloride - metabolism; Spores, Bacterial - cytology; Systematics; Temperature; Water Microbiology; Iran; Bacillales; Halophily; Taxonomy; Bacillus; Lakes; Bacteria; New species; Bacillaceae; Salinity; Hypersaline environment
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.010090-0

1 Extremophiles Laboratory, Department of Microbiology, Faculty of Biology, College of Science, University of Tehran, Tehran, Iran
2 Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain
3 Department of Biology, Islamic Azad University, Science and Research Campus, Tehran, Iran
Correspondence A. Ventosa ventosa{at}us.es
A Gram-positive, moderately halophilic, endospore-forming bacterium, designated strain HS136 T , was isolated from the hypersaline lake Howz-Soltan in Iran. Cells were motile rods, producing ellipsoidal endospores at a central–subterminal position in non-swollen sporangia. Strain HS136 T , a strictly aerobic bacterium, grew between pH 7.0 and 10.0 (optimal growth at pH 8.0–8.5), between 25 and 45 °C (optimal growth at 40 °C) and at salinities of 5–20 % (w/v) NaCl, growing optimally at 10 % (w/v) NaCl. On the basis of 16S rRNA gene sequence analysis, strain HS136 T was shown to belong to the genus Bacillus within the phylum Firmicutes and showed closest phylogenetic similarity to Bacillus salarius BH169 T (95.2 %) and Bacillus qingdaonensis CM1 T (94.5 %). The DNA G+C content of this new isolate was 37.1 mol%. The major cellular fatty acids of strain HS136 T were iso-C 15 : 0 , anteiso-C 15 : 0 and anteiso-C 17 : 0 , and its polar lipid pattern consisted of phosphatidylglycerol and diphosphatidylglycerol. The isoprenoid quinone was MK-7. The peptidoglycan type is A1 , with meso-diaminopimelic acid as the diagnostic diamino acid. On the basis of polyphasic evidence from this study, Bacillus persepolensis sp. nov. is proposed, with strain HS136 T (=CCM 7595 T =DSM 21632 T =JCM 15720 T =LMG 25222 T ) as the type strain.
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain HS136 T is FM244839 .