Fine mapping of powdery mildew resistance gene PmTm4 in wheat using comparative genomics

• Published in: Journal of Integrative Agriculture Vol. 16; no. 3; pp. 540 - 550
• Main Authors: XIE, Jing-zhong, WANG, Li-li, WANG, Yong, ZHANG, Huai-zhi, ZHOU, Sheng-hui, WU, Qiu-hong, CHEN, Yong-xing, WANG, Zhen-zhong, WANG, Guo-xin, ZHANG, De-yun, ZHANG, Yan, HU, Tie-zhu, LIU, Zhi-yong
• Format: Journal Article
• Language: English
• Published: State Key Laboratory for Agrobiotechnology,China Agricultural University,Beijing 100193,P.R.China%Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,P.R.China%China Rural Technology Development Center,Beijing 100045,P.R.China%College of Life Science and Technology,Henan Institute of Science and Technology,Xinxiang 453003,P.R.China%State Key Laboratory for Agrobiotechnology,China Agricultural University,Beijing 100193,P.R.China 01-03-2017; Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,P.R.China; Elsevier
• Subjects: comparative genomic analysis; genetic mapping; PmTm4; powdery mildew resistance gene; 同源序列; 小麦品种; 小麦病害; 应用程序; 抗白粉病基因; 比较基因组学; 精细定位; 遗传图谱构建; comparative genomic analysis; genetic mapping; PmTm4; powdery mildew resistance gene
• ISSN: 2095-3119; 2352-3425
• DOI: 10.1016/S2095-3119(16)61377-1

Powdery mildew,caused by Blumeria graminis f.sp.tritici,is one of the most severe wheat diseases.Mining powdery mildew resistance genes in wheat cultivars and their appliance in breeding program is a promising way to control this disease.Genetic analysis revealed that a single dominant resistance gene named PmTm4 originated from Chinese wheat line Tangmai 4 confers resistance to prevailing isolates of B.graminis f.sp.tritici isolate E09.Detailed comparative genomics analyses helped to develop closely linked markers to PmTm4 and a fine genetic map was constructed using large F2population,in which PmTm4 was located into a 0.66-c M genetic interval.The orthologous subgenome region of PmTm4in Aegilops tauschii was identified,and two resistance gene analogs（RGA）were characterized from the corresponding sequence scaffolds of Ae.tauschii draft assembly.The closely linked markers and identified Ae.tauschii orthologs in the mapping interval provide an entry point for chromosome landing and map-based cloning of PmTm4.