Effect of Phosphorylation on Activities of Rap1A to Interact with Raf-1 and to Suppress Ras-dependent Raf-1 Activation

• Published in: The Journal of biological chemistry Vol. 274; no. 1; pp. 48 - 51
• Main Authors: Hu, Chang-Deng, Kariya, Ken-ichi, Okada, Tomoyo, Qi, Xiaodong, Song, Chunhua, Kataoka, Tohru
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-01-1999; American Society for Biochemistry and Molecular Biology
• Subjects: Amino Acid Sequence; Animals; COS Cells; GTP-Binding Proteins - chemistry; GTP-Binding Proteins - genetics; GTP-Binding Proteins - metabolism; Molecular Sequence Data; Mutation; Phosphorylation; Protein Binding; Proto-Oncogene Proteins c-raf - metabolism; rap GTP-Binding Proteins; ras Proteins - metabolism; Recombinant Proteins - metabolism; Serine - metabolism
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.274.1.48

Rap1A is phosphorylated by cAMP-dependent protein kinase (PKA), and this phosphorylation has been shown to modulate its interaction with other proteins. However, it is not known whether Rap1A phosphorylation is involved in regulation of its cellular functions, including suppression of Ras-dependent Raf-1 activation. We have previously shown that this suppressive activity of Rap1A is attributable to its greatly enhanced ability to bind to the cysteine-rich region (CRR, residues 152–184) of Raf-1 compared with that of Ras. Here, we show that phosphorylation of Rap1A by PKA abolished its binding activity to CRR. Furthermore, a mutant Rap1A(S180E), whose sole PKA phosphorylation residue, Ser-180, was substituted by an acidic residue, Glu, to mimic its phosphorylated form, failed to suppress Ras-dependent Raf-1 activation in COS-7 cells. These results indicate that the CRR binding activity and the Ras-suppressive function of Rap1A can be modulated through phosphorylation and suggest that Rap1A may function as a PKA-dependent regulator of Raf-1 activation, not merely as a suppressor.