Assessment of sensitivity and specificity of first, second, and third generation EIA for the detection of antibodies to HIV-1 in oral fluid

• Published in: Journal of virological methods Vol. 159; no. 1; pp. 119 - 121
• Main Authors: Louie, Brian, Lei, John, Liska, Sally, Dowling, Teri, Pandori, Mark W.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 01-07-2009; Elsevier
• Subjects: Antibodies, Viral - blood; Antibodies, Viral - immunology; Antibodies, Viral - isolation & purification; Antibody; Biological and medical sciences; EIA; Fundamental and applied biological sciences. Psychology; HIV Infections - blood; HIV Infections - diagnosis; HIV Infections - immunology; HIV-1; HIV-1 - immunology; Human immunodeficiency virus 1; Humans; Immunoenzyme Techniques - methods; Microbiology; Oral fluid; Reagent Kits, Diagnostic; Saliva - immunology; Sensitivity; Sensitivity and Specificity; Signal-to-cutoff; Techniques used in virology; Virology; Western blot; HIV-1; Western blot; Signal-to-cutoff; Sensitivity; Antibody; Oral fluid; EIA; Microbiology; HIV-1 virus; Retroviridae; Lentivirus; Virology; Virus; Specificity; Immunoblotting assay; Human immunodeficiency virus; Detection
• ISSN: 0166-0934; 1879-0984
• DOI: 10.1016/j.jviromet.2009.02.016

The performances of three blood-based immunoassays test kits were compared with regard to their ability to detect HIV-1 antibody in oral fluid. It was found that these three kits differ in their ability to detect HIV-1 antibody. Notably, a third generation EIA which has been shown to possess superior sensitivity for antibody detection in plasma appears to possess no sensitivity advantage for detecting HIV-1 antibody in oral fluid.