Effects of endothelin-3 on intestinal ion transport

• Published in: Peptides (New York, N.Y. : 1980) Vol. 22; no. 12; pp. 2069 - 2075
• Main Authors: González Bosc, L.V, Majowicz, M.P, Ortiz, M.C, Vidal, N.A
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-12-2001
• Subjects: Animals; Endothelin 3; Endothelin-3 - pharmacology; Intestine, Small - drug effects; Intestine, Small - enzymology; Intestine, Small - metabolism; Ion Transport; Male; NADPH Dehydrogenase - metabolism; Nitrates - urine; Nitric oxide; Nitric Oxide - physiology; Nitrites - urine; Rats; Rats, Wistar; SGLT1; Small intestine; Endothelin 3; Ion transport; Small intestine; Nitric oxide; SGLT1
• ISSN: 0196-9781; 1873-5169
• DOI: 10.1016/S0196-9781(01)00554-X

We investigated the effects of endothelin 3 (ET-3) on electrolyte transport in rat small intestine using a voltage clamp technique in Ussing’s chamber. ET-3 diminished potential difference (PD) and short circuit current (Isc). ET-3 did not affect PD or Isc in low Na
+ and/or D-glucose-free medium. Phloridzine (an inhibitor of sodium-glucose cotransporter [SGLT1]) pretreatment abolished the effect of ET-3 on Isc. Methylene blue (a soluble guanylate cyclase inhibitor) or N-nitro-L-arginine methyl ester (a NOS inhibitor) pretreatment delayed the effect of ET-3 on PD and Isc. ET-3 enhanced NOS activity on enterocytes and systemic NO production. Then, ET-3 could inhibit SGLT1 with the participation of NO.