Molecular Detection of Respiratory Tract Viruses in Chickens at the Point of Need by Loop-Mediated Isothermal Amplification (LAMP)

• Published in: Viruses Vol. 16; no. 8; p. 1248
• Main Authors: El-Tholoth, Mohamed, Bau, Haim H
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 03-08-2024; MDPI
• Subjects: Animals; Causes of; chickens; Chickens - virology; Climate change; Contaminants; Diseases; DNA polymerase; Energy consumption; Farms; Food chains; Food security; Gene amplification; Genetic aspects; Genetic screening; Health aspects; Identification and classification; Influenza; LAMP; Methods; molecular diagnosis; Molecular Diagnostic Techniques - methods; Molecular Diagnostic Techniques - veterinary; Mortality; Nucleic Acid Amplification Techniques - methods; Nucleic Acid Amplification Techniques - veterinary; Pathogens; point of need; Polymerase chain reaction; Poultry; Poultry Diseases - diagnosis; Poultry Diseases - virology; Power consumption; rapid detection; Respiration; Respiratory diseases; Respiratory tract diseases; Respiratory tract infections; Respiratory Tract Infections - diagnosis; Respiratory Tract Infections - veterinary; Respiratory Tract Infections - virology; Review; Sensitivity and Specificity; Temperature; Temperature requirements; Temperature tolerance; Thermal cycling; Viruses; Viruses - classification; Viruses - genetics; Viruses - isolation & purification; Egypt; chickens; point of need; LAMP; rapid detection; respiratory diseases; molecular diagnosis
• ISSN: 1999-4915; 1999-4915
• DOI: 10.3390/v16081248

Accurate and timely molecular diagnosis of respiratory diseases in chickens is essential for implementing effective control measures, preventing the spread of diseases within poultry flocks, minimizing economic loss, and guarding food security. Traditional molecular diagnostic methods like polymerase chain reaction (PCR) require expensive equipment and trained personnel, limiting their use to centralized labs with a significant delay between sample collection and results. Loop-mediated isothermal amplification (LAMP) of nucleic acids offers an attractive alternative for detecting respiratory viruses in broiler chickens with sensitivity comparable to that of PCR. LAMP's main advantages over PCR are its constant incubation temperature (∼65 °C), high amplification efficiency, and contaminant tolerance, which reduce equipment complexity, cost, and power consumption and enable instrument-free tests. This review highlights effective LAMP methods and variants that have been developed for detecting respiratory viruses in chickens at the point of need.